The invention discloses a traditional Chinese medicinal injection for preventing and treating a chronic Aleutian disease of a mink and a preparation method thereof. The traditional Chinese medicinal injection is prepared from the following components as raw materials: 5-15 parts of aspleniaceae, 8-20 parts of madder, 10-20 parts of berchemia sinica, 10-20 parts of serissa serissoides, 10-20 parts of kochia shoot, 5-15 parts of farfugium japonicum, 6-12 parts of bighead atractylodes rhizome, 10-30 parts of corn stigma, 4-8 parts of fennel, 4-10 parts of lindera aggregata, 10-20 parts of mountain ebony, 6-9 parts of pummelo seed, 10-25 parts of russula virescens, 10-30 parts of ventilago leiocarpa benth, 10-20 parts of argentina anserina, 5-10 parts of argentina anserina, 5-10 parts of lucid ganoderma, 5-15 parts of ginseng, 5-15 parts of polygonum multiflorum and 6-10 parts of liquorice. The traditional Chinese medicinal injection is a pure traditional Chinese medicinal preparation, is non-toxic to the mink and convenient to use, can take effect quickly, has a good therapeutic effect on the chronic Aleutian disease of the mink, can enhance the immunity of the mink, and is conducive to improving economic benefits of a farmer.