The invention discloses a cultivation method of organic shiitake. The method includes the steps of material preparation, blending, bagging, sterilization, cooling, inoculation, culture, fruiting management and harvesting. Prepared materials include, by weight, 50-65 parts of corn cobs, 20-30 parts of straw pieces, 20-25 parts of hard and mixed wood dust, 5-8 parts of hemp stems, 5-10 parts of cottonseed hulls, 6-15 parts of rice bran, 3-7 parts of corn flour, 3-5 parts of soybean meal, 5-8 parts of wheat meal, 5-10 parts of bean cakes, 1-2 parts of lime powder and 1-3 parts of sugar. According to the method, by taking the measures of selecting a fine strain, mastering the optimum cultivation season, adopting high-quality raw materials and the optimum formula, improving the cultivation pattern and the like, the yield and quality of the shiitake are further improved, the production cycle is shortened, and the produced shiitake is plump and sturdy in flesh, aromatic in flavor, good in taste, large in size and convenient to pick; besides, the shiitake is excellent in quality and high in yield, the yield is increased, manpower and material resources are saved accordingly, and economic benefits are improved.