2results about How to "Significant selectivity" patented technology

Provided are an isolated recombinant oncolytic poxvirus capable of being regulated and controlled by microRNA and a use thereof. The isolated recombinant oncolytic poxvirus can be regulated and controlled by microRNA, and the expression level of the microRNA in tumor cells of a mammal is lower than that in normal cells of the same mammal. A target sequence of the microRNA is integrated in a 3'UTR region of an E10R gene in a recombinant oncolytic poxvirus genome.

The application discloses a method for preparing a chiral biphenyl-substituted propanol compound by using alcohol dehydrogenase, and the alcohol dehydrogenase is used to catalyze a biphenyl-substituted propanone reaction to generate a target product. The alcohol dehydrogenase is derived from Leifsonia sp., the amino acid sequence of the alcohol dehydrogenase is shown as SEQ ID NO. 1, and the nucleotide sequence of the alcohol dehydrogenase is shown as SEQ ID NO. 2. Under the optimized alcohol dehydrogenase catalytic reaction condition, (S)-1-(4-biphenyl)-3-chloro-2-propanol is synthesized, the substrate concentration is 100 g / L, the reaction conversion rate is greater than 99%, and the chiral purity is greater than 99.5% ee, and the method has good industrial application value. R is hydrogen, halogen, alkyl, alkoxy, hydroxyl or the like.