35 results about "Ampullaria crossean" patented technology

The invention belongs to the technical field of utilization of ampullariidae killing plant resources and particularly relates to a method for killing ampullariidae by using mikania micrantha. The method for killing the ampullariidae by using the mikania micrantha comprises the following steps: throwing mikania micrantha powder into water containing the ampullariidae for directly killing the ampullariidae, wherein the using amount of the mikania micrantha powder is 005-15 g / L. The method for killing the ampullariidae by using the mikania micrantha has the advantage that the plant material mikania micrantha is a common invasive plant in southern China, is easy to obtain and can effectively kill the ampullariidae at a certain using amount.

The invention discloses a traditional Chinese medicine residue bio-organic fertilizer and a preparation method thereof, and belongs to the technical field of the fertilizer preparation. The traditional Chinese medicine residue bio-organic fertilizer comprises the following raw materials by weight: 150-450 parts of traditional Chinese medicine residue, 50-80 parts of chicken manure, 40-70 parts ofegg shell, 65-95 parts of crop straw, 45-85 parts of a soil conditioner, 10-20 parts of ampullaria crossean powder, 0.6-2 parts of compound bacteria, 1.5-3 parts of polyoxyethylene, 1-2.5 parts of ammonium polybutylene, 0.3-0.6 parts of brassinolide, and 0.2-0.4 parts of chitosan oligosaccharide. The traditional Chinese medicine residue bio-organic fertilizer is prepared by the steps of fermenting, concentrating, smashing, pelleting and so on. The fertilizer is capable of effectively preventing diseases and insect pests, balancing the nutrition, promoting the rapid and healthy growth of corns,improving the protein and vitamin content and the yield of the corns, and reducing the disease and pest rate.

The invention discloses an electric ship for removing ampullaria gigas eggs. The electric ship comprises a ship body; an egg receiving mechanism and a crushing mechanism are arranged at the upper end of the ship body; front-back symmetrical baffles are fixedly arranged at the front end and the rear end of the ship body; a first fixed plate is fixedly arranged at the upper end of the ship body; and a seventh rotating shaft, a fourth hinged shaft and a fifth hinged shaft are sequentially and rotationally arranged on the first fixed plate from left to right. The electric ship is simple in structure, easy and convenient to operate and relatively high in integration degree; the electric ship can patrol along a river bank automatically; when a camera finds that ampullaria gigas exists on the river bank, the orientation of a ship head can be adjusted; the ampullaria gigas eggs attached to the river bank are scraped off, the scraped ampullaria gigas eggs are crushed, and the crushed ampullaria gigas eggs can be poured back into water to become fish feed; and theampullaria gigas eggs are removed byregular cruising of the electric ship, so that the appearance of the river bank can be effectively kept, and the ampullaria gigas can be effectively prevented from being overrun.

The invention discloses a preventing and controlling method of ampullaria crossean. The preventing and controlling method is characterized by comprising the steps of 1, invasion interception, wherein a block is arranged at a water inlet of a water pond, and plants with a repellent effect of ampullaria crossean are planted around the water pond; 2, trapping and killing, wherein ampullaria crossean catching nets are arranged in the middle and the periphery of the water pond, and plants with the attracting effect of ampullaria crossean are planted around the ampullaria crossean catching nets; 3, plant secondary substance control, wherein a powder mixture of nerium indicum and hibiscus is thrown at the low-lying position of the water pond. According to the preventing and controlling method of ampullaria crossean, by utilizing the ecological habit of ampullaria crossean to conduct prevention and control, a better preventing and controlling effect is achieved; the plant source secondary substance is adopted to prevent and control ampullaria crossean, and the preventing and treating method is safe to the environment and other aquatic life, is an environment-friendly and efficient preventing and controlling method, and is suitable for being applied and popularized in prevention and control of ampullaria crossean.

The invention provides novel multifunctional cellulase derived from Ampullaria crossean, polynucleotide for coding the enzyme, and a method for producing the enzyme by DNA recombination technology. The invention also discloses a vector and host cells containing the multifunctional cellulase, and the use in production of monosaccharides and glucose.

The invention relates to a pomacea canaliculata egg mass monitoring method based on a wireless remote control electric ship, which is characterized by comprising the following steps of: 1, manufacturing the wireless remote control electric ship; 2, fixing a camera; 3, connecting the camera with the mobile phone; 4, performing ampullaria gigas egg mass image acquisition: starting the electric ship and the camera, and acquiring the ampullaria gigas egg mass image; and 5, performing ampullaria gigas egg mass image analysis: importing the ampullaria gigas image data into a computer, opening Photoshop software to splice single ampullaria gigas egg mass images, and synthesizing an integral ampullaria gigas egg mass area image; and performing multi-point selection on the ampullaria gigas egg mass image by using a magic wand tool on the whole image of the ampullaria gigas egg mass area, outputting an ampullaria gigas egg mass distribution map, and observing the size and the number of the egg mass from the ampullaria gigas egg mass distribution map. The invention provides the pomacea canaliculata egg mass monitoring method based on the wireless remote control electric ship, and solves the problems of high risk and poor accuracy existing in manual onsite ocularization egg mass quantity and east-west estimation.

The invention discloses chicken feed. The chicken feed comprises, by weight, 50-60 parts of corn, 2-6 parts of sweet potatoes, 1-5 parts of pumpkins, 3-7 parts of rice, 5-10 parts of sweet potato seedling powder, 5-10 parts of peanut seedling powder, 2-6 parts of millet bran, 0.5-1.5 parts of limestone powder, 4-8 parts of ampullaria crossean, 3-9 parts of peanut shell powder, 5-10 parts of earthworms, 0.1-0.3 part of jujube powder, 0.5-1 part of lard oil, 0.5-1 part of calcium hydrophosphate, 0.3-0.5 part of sodium chloride, 1-3 parts of lysine, 0.5-1 part of methionine, 0.01-0.03 part of glycine, 0.2-0.4 part of cordyceps militaris, 0.1-0.3 part of wormwood, 0.02-0.04 part of talcum powder and 0.05-0.1 part of citric acid. The chicken feed has the advantages of being low in cost and good in quality.

The invention relates to a method for detecting pomacea canaliculata and controlling dosage by using an unmanned aerial vehicle. The method aims at monitoring and killing invading organisms, namely ampullaria gigas, in an agricultural rice field. The distribution of the ampullaria gigas in an area to be searched and killed is monitored through the unmanned aerial vehicle; the unmanned aerial vehicle can go deep into a farmland into which people cannot conveniently enter; the distribution density of the ampullaria gigas is speculated according to the number of the detected ampullaria gigas eggmasses, so that ampullaria gigas killing agents with different concentrations are selected, the purpose of accurate prevention and treatment is achieved, the prevention and treatment effect is achieved under the condition that the ampullaria gigas killing agents are used as little as possible, the cost is reduced, and the environment is protected.

The invention discloses chicken breeding feed. The chicken breeding feed comprises, by weight, 55-56 parts of corn, 2-4 parts of sweet potatoes, 2-5 parts of pumpkins, 5-7 parts of rice, 7-9 parts of sweet potato seedling powder, 5-8 parts of peanut seedling powder, 3-5 parts of millet bran, 0.7-1.1 parts of limestone powder, 2-7 parts of ampullaria crossean, 2-5 parts of peanut shell powder, 6-9 parts of earthworms, 5-8 parts of feather meal, 0.2-0.5 part of jujube powder, 0.5-0.7 part of lard oil, 0.5-0.7 part of calcium hydrophosphate, 0.2-0.6 part of sodium chloride, 1-3 parts of lysine, 0.5-0.9 part of methionine, 0.02-0.04 part of glycine, 0.3-0.6 part of cordyceps militaris, 0.1-0.4 part of wormwood, 0.01-0.04 part of talcum powder and 0.05-0.07 part of citric acid. The chicken breeding feed has the advantages of being low in cost and good in quality.

The invention discloses a TPR1 gene related to low-temperature tolerance of pomacea canaliculata, an encoded protein and application of the TPR1 gene, and belongs to the technical field of biology. The method is rapid, effective and high in repeatability, and is an important supplement to the TPR1 gene family. The pomacea canaliculata is an important invasive organism, development of novel biopesticides is facilitated, and the low-temperature tolerance of the pomacea canaliculata is reduced by blocking expression of the TPR1 gene in the pomacea canaliculata, so that the purpose of controlling further north invasion of the pomacea canaliculata is achieved. Meanwhile, by interfering the expression of the TPR1 gene, the hatching rate of the eggs of the ampullaria gigas can be remarkably reduced, the hatching duration of the eggs can be prolonged, and therefore the harm of invasion of the ampullaria gigas is reduced. The method is of great significance to research and determination of biology, ecology and novel insecticides of the ampullaria gigas and research on a resistance mechanism of the ampullaria gigas to low-temperature tolerance.

The invention provides novel multifunctional cellulase derived from Ampullaria crossean, polynucleotide for coding the enzyme, and a method for producing the enzyme by DNA recombination technology. The invention also discloses a vector and host cells containing the multifunctional cellulase, and the use in production of monosaccharides and glucose.

The invention relates to a cellulase orthogenesis method of ampullaria crossean. The steps are as follows: step one, acquiring cellulase genes of ampullaria crossean: firstly extracting the total mRNA of ampullaria crossean; secondly conducting reverse transcription to amplify cDNA; then amplifying the cellulase genes of ampullaria crossean through PCR; and obtaining the cellulase gene fragments of ampullaria crossean; step two, cloning and sequencing: cloning PCR products and connecting the PCR products with pMD18-T carrier containing T-end; preparation and conversion of competent cell, and adopting a CaCL 2 method to prepare the competent cell; screening of positive clones, namely using X-ga1 and IPTG to primarily screen clones; extracting plasmid by an alkaline process; and sequencing; and step three: site-mutagenesis: using professional software and websites of molecular biology to conduct analytical prediction of functions and determine amino acid needing transforming; adopting a kit to conduct site-mutagenesis in the direction transformation of cellulase protein; and finally sequencing and determining. The method improves the activity of cellulase of ampullaria crossean, as well as the degradation action of cellulase of ampullaria crossean on cellulose.

The invention discloses a vegetable crop for efficiently trapping ampullaria gigas and belongs to the technical field of trapping of foreign species. The vegetable crop for efficiently trapping the ampullaria gigas is either pakchoi or duckweed. The vegetable crop has the beneficial effects that the ampullaria gigas is trapped according to the fondness of the ampullaria gigas to foods, the purpose of reducing damage caused by the ampullaria gigas is achieved, ampullaria gigas populations can be effectively trapped and controlled, and the ecological environment of farmland can be protected.

The invention belongs to the technical field of ampullaria gigas, and particularly relates to an adult ampullaria gigas trapping device suitable for paddy fields. The adult ampullaria gigas trapping device comprises a bottom plate, wherein an arc-shaped connecting plate is arranged around the surface of the bottom plate, one end of the arc-shaped connecting plate is connected with a trapping shell, the surface of the bottom of the trapping shell is connected with a trapping and killing barrel, a trapping shell inlet is formed in the inner wall of the trapping shell, and the position of the trapping shell inlet is located over the arc-shaped connecting plate. By means of a rotating rod, an L-shaped rotating rod, a temporary standing disc and a salt storage shell, the ampullaria gigas falls into salt water in the trapping and killing barrel, water in the ampullaria gigas can permeate from the body with low salt content to the position with high salt content outside the body, the salt water enables the ampullaria gigas to lose water to die through dehydration, due to the fact that the density of the water is smaller than that of the salt water, the water in the ampullaria gigas is not prone to being fused with the salt water, water can be located on the salt water, when the ampullaria gigas is trapped into the trapping and killing barrel, the water can overflow from an overflow cavity when the water level line exceeds the water outlet, and nutrient substances in the water can feed the rice back.