47 results about "Ampullaria crossean" patented technology

The invention discloses a biological organic fertilizer, and belongs to the technical field of fertilizer preparing. The biological organic fertilizer is prepared from, by weight, cassava residues, beer residues, peat soil, traditional Chinese medicine residues, tea bran, animal waste, microelements, ampullaria crossean powder, a fermentation preparation and a conditioning agent. A preparing method of the biological organic fertilizer includes the steps of raw-material pretreating, drying, stack fermenting, fermenting, packaging and the like. According to the biological organic fertilizer, waste such as the cassava residues and the peat soil are effectively used, the physical and chemical properties of the cassava residues are changed by optimizing the fermentation method, the cassava residues are degraded into micromolecule substances such as monosaccharide and amino acid, absorption and utilization of crops can be better achieved, the ecological environment can be improved, and the production cost of the fertilizer can also be reduced.

The invention belongs to the technical field of utilization of ampullariidae killing plant resources and particularly relates to a method for killing ampullariidae by using mikania micrantha. The method for killing the ampullariidae by using the mikania micrantha comprises the following steps: throwing mikania micrantha powder into water containing the ampullariidae for directly killing the ampullariidae, wherein the using amount of the mikania micrantha powder is 005-15 g / L. The method for killing the ampullariidae by using the mikania micrantha has the advantage that the plant material mikania micrantha is a common invasive plant in southern China, is easy to obtain and can effectively kill the ampullariidae at a certain using amount.

The invention discloses a Truogx sinensis feed and its preparation method and belongs to the technical field of testudinate feed processing. The feed provided by the invention comprises the following raw materials, by weight, ampullaria crossean, livestock leftovers, earthworm, locust, corn, sweet potato, dimethyl-beta-dimethylpropiothetin, betaine, amino acid, nucleotide and a Chinese herbal medicine phagostimulant. The feed is prepared by steps of crushing, sieving, mixing, granulation, drying, cooling, packaging and the like. The phagostimulant in the feed is an efficient, safe and natural additive prepared from a plurality of Chinese herbal medicines, integrates efficacy of multiple Chinese herbal medicines, enhances disease resistance of Truogx sinensis and raises survival rate. The feed provided by the invention has comprehensive nutrients, has strong palatability, and can effectively raise food consumption and growth speed of Truogx sinensis and shorten growth cycle. The raw materials of the feed have wide sources and are low-cost. Thus, making costs of the feed are reduced, and production benefit is enhanced.

The invention relates to a high-density artificial culturing method of wide body leeches. The method comprises the steps that 1. a culturing pond is built; 2. culturing matrixes are placed; 3. ampullariagigas and / or escargots are subjected to mixed culturing; 4. the young is put in; 5. fodder feeding is carried out; 5. leech pond dividing is carried out; 7. water quality management is carried out; and 8. disease preventing and curing management is carried out. The high-density culturing condition of the wide body leeches can be well controlled, observing and managing of the wide body leeches are convenient, leech disease preventing and treatment management is enhanced, the high yield of 500-600 kilograms per acre can be achieved, and the economic benefit of wide body leech artificial culturing is increased.

The invention discloses a traditional Chinese medicine residue bio-organic fertilizer and a preparation method thereof, and belongs to the technical field of the fertilizer preparation. The traditional Chinese medicine residue bio-organic fertilizer comprises the following raw materials by weight: 150-450 parts of traditional Chinese medicine residue, 50-80 parts of chicken manure, 40-70 parts ofegg shell, 65-95 parts of crop straw, 45-85 parts of a soil conditioner, 10-20 parts of ampullaria crossean powder, 0.6-2 parts of compound bacteria, 1.5-3 parts of polyoxyethylene, 1-2.5 parts of ammonium polybutylene, 0.3-0.6 parts of brassinolide, and 0.2-0.4 parts of chitosan oligosaccharide. The traditional Chinese medicine residue bio-organic fertilizer is prepared by the steps of fermenting, concentrating, smashing, pelleting and so on. The fertilizer is capable of effectively preventing diseases and insect pests, balancing the nutrition, promoting the rapid and healthy growth of corns,improving the protein and vitamin content and the yield of the corns, and reducing the disease and pest rate.

The invention discloses an electric ship for removing ampullaria gigas eggs. The electric ship comprises a ship body; an egg receiving mechanism and a crushing mechanism are arranged at the upper end of the ship body; front-back symmetrical baffles are fixedly arranged at the front end and the rear end of the ship body; a first fixed plate is fixedly arranged at the upper end of the ship body; and a seventh rotating shaft, a fourth hinged shaft and a fifth hinged shaft are sequentially and rotationally arranged on the first fixed plate from left to right. The electric ship is simple in structure, easy and convenient to operate and relatively high in integration degree; the electric ship can patrol along a river bank automatically; when a camera finds that ampullaria gigas exists on the river bank, the orientation of a ship head can be adjusted; the ampullaria gigas eggs attached to the river bank are scraped off, the scraped ampullaria gigas eggs are crushed, and the crushed ampullaria gigas eggs can be poured back into water to become fish feed; and theampullaria gigas eggs are removed byregular cruising of the electric ship, so that the appearance of the river bank can be effectively kept, and the ampullaria gigas can be effectively prevented from being overrun.

The invention relates to a cellulase orthogenesis method of ampullaria crossean. The steps are as follows: step one, acquiring cellulase genes of ampullaria crossean: firstly extracting the total mRNA of ampullaria crossean; secondly conducting reverse transcription to amplify cDNA; then amplifying the cellulase genes of ampullaria crossean through PCR; and obtaining the cellulase gene fragments of ampullaria crossean; step two, cloning and sequencing: cloning PCR products and connecting the PCR products with pMD18-T carrier containing T-end; preparation and conversion of competent cell, and adopting a CaCL 2 method to prepare the competent cell; screening of positive clones, namely using X-ga1 and IPTG to primarily screen clones; extracting plasmid by an alkaline process; and sequencing; and step three: site-mutagenesis: using professional software and websites of molecular biology to conduct analytical prediction of functions and determine amino acid needing transforming; adopting a kit to conduct site-mutagenesis in the direction transformation of cellulase protein; and finally sequencing and determining. The method improves the activity of cellulase of ampullaria crossean, as well as the degradation action of cellulase of ampullaria crossean on cellulose.

The invention discloses a breeding method for a laying hen. The method comprises the following steps: firstly, diapausing hatching eggs and desalting hormone and antibiotics, then putting onto a hatching tray, and hatching; secondly, feeding chicks hatched for 1-5 days with a mixture of green vegetable size and rice bran for three times everyday, enabling chicks with the age of 6-20 days to live in thick growth of grass with a hen after hatching chicks together in the daytime, and uniformly scattering smashed rice grains in a feeding region in the morning everyday and feeding with a mixture ofgreen vegetable size and rice bran in the afternoon everyday; thirdly, classifying cocks and hens in the chicks with the age of 20-25 days, and taking the classified hens with the age of more than 20days as laying hens, uniformly scattering the rice grains and unhusked rice in the feeding region in the morning everyday, and feeding a mixture of rice, corn kernels, banana peel size and rice branin the afternoon everyday; and fourthly, feeding the laying hens with the age of more than 140 days with a mixture of ampullaria crossean meat and aloe juice at noon everyday, and keeping 6-10 hours of sunshine duration everyday. The method disclosed by the invention can effectively increase egg yield of the laying hens and improve quality of native eggs.

The invention discloses a production method of NAD fermentation liquor. The method includes the steps of S1, well mixing crushed ampullaria crossean soft tissue and bacillus subtilis neutral proteinase, performing enzymolysis, filtering, and taking supernate and drying to obtain ampullaria crossean soft tissue protein dissolved matter; S2, well mixing cassava starch and water, adding alpha-amylase for liquefaction, and then adding saccharification liquid for saccharification to obtain cassava saccharification liquid; S3, well mixing the ampullaria crossean soft tissue protein dissolved matter and the cassava saccharification liquid to obtain solution A, adding vitamin complex liquid and well mixing to obtain solution B, sterilizing, cooling to room temperature, adding fermentation strains, heating to 28-32 DEG C, and keeping the temperature for 36-48 hours to obtain the NAD fermentation liquor. The method has the advantages that NAD accumulation amount in the fermentation liquor is increased greatly and can reach 4.5-6.5g / l, and the method is cheap in raw materials, easy in raw material obtaining, low in cost, simple to operate and suitable for industrial production.

The invention discloses a preventing and controlling method of ampullaria crossean. The preventing and controlling method is characterized by comprising the steps of 1, invasion interception, wherein a block is arranged at a water inlet of a water pond, and plants with a repellent effect of ampullaria crossean are planted around the water pond; 2, trapping and killing, wherein ampullaria crossean catching nets are arranged in the middle and the periphery of the water pond, and plants with the attracting effect of ampullaria crossean are planted around the ampullaria crossean catching nets; 3, plant secondary substance control, wherein a powder mixture of nerium indicum and hibiscus is thrown at the low-lying position of the water pond. According to the preventing and controlling method of ampullaria crossean, by utilizing the ecological habit of ampullaria crossean to conduct prevention and control, a better preventing and controlling effect is achieved; the plant source secondary substance is adopted to prevent and control ampullaria crossean, and the preventing and treating method is safe to the environment and other aquatic life, is an environment-friendly and efficient preventing and controlling method, and is suitable for being applied and popularized in prevention and control of ampullaria crossean.

The invention relates to a floating type ampullaria gigas egg trapping device changing along with the water level. The floating type ampullaria gigas egg trapping device comprises a trap, a floating ring and a collector. The trapper is in the shape of a cylinder or a regular polygon prism and comprises a shell and a one-way movable door. A plurality of window openings are formed in the periphery of the shell, a pair of door seats are arranged in the middles of the two sides of each window opening, and the one-way movable door is hinged to the door seats through a transverse door shaft in the middle. An annular partition plate used for preventing the lower portion of the one-way movable door from turning inwards is arranged on the periphery of the bottom of the inner side of the one-way movable door. The weight of the lower end of the one-way movable door is larger than that of the upper end, and the one-way movable door is naturally closed under the action of the self weight of the lower end in a natural state; the floating ring is fixedly installed on the lower portion of the trap and used for providing buoyancy. The collector is fixedly mounted at the lower part of the floating ring; the trapper, the floating ring and the collector are communicated in the vertical direction.

The invention relates to constitutive expression and purification of ampullaria gigas endogenous cellulase EG27I in pichia yeast. Through the construction of recombinant expression plasmid of ampullaria gigas endogenous cellulase EG27 gene, obtained is a method for highly expressing constitutive of Pichia pastoris and purifying ampullaria gigas endogenous cellulase EG27I.

The invention provides novel multifunctional cellulase derived from Ampullaria crossean, polynucleotide for coding the enzyme, and a method for producing the enzyme by DNA recombination technology. The invention also discloses a vector and host cells containing the multifunctional cellulase, and the use in production of monosaccharides and glucose.

The invention discloses a specific primer pair for identifying spotted ampullaria gigas in an assisted manner and application of the specific primer pair.The specific primer pair provided by the invention consists of a DNA sequence shown in a sequence 1 of a sequence table and a DNA sequence shown in a sequence 2 of the sequence table.By the specific primer pair disclosed by the invention, identification of the spotted ampullaria gigas can be carried out in the assisted manner from the molecular level, and is not affected by individual development states of specimens or integrity of samples, and the specific primer pair has the advantages of high efficiency, convenience and accuracy.The problem of type identification of the spotted ampullaria gigas which perplexes plant protection and port quarantine inspection departments for a long time is solved.The specific primer pair can be widely used for plant protection departments, quarantine departments and institutions for researching ampullaria gigas.

The invention discloses a fertilizer for promoting growth of rice, which is prepared from the following component raw materials in parts by weight: 100 to 200 parts of nitrogen fertilizer, 40 to 80 parts of phosphorus fertilizer, 10 to 20 parts of zinc fertilizer, 10 to 20 parts of dicranopteris pedata, 3 to 5 parts of algae and 0.1 to 0.7 part of tea polyphenol. According to the fertilizer for promoting growth of rice, which is disclosed by the invention, the dicranopteris pedata, the algae and the tea polyphenol are adopted to interact with each other, so that fertilizer efficiency of the product can be improved and meanwhile, adsorption of rice for effective components in the fertilizer is improved; furthermore, the tea polyphenol can effectively remove ampullaria crossean in paddy fields and has no side effects on rice and the environment; the dicranopteris pedata is a wild plant, is easy to grow, is great in quantity, and can effectively reduce production cost as the raw material for synthesizing the fertilizer for promoting growth of rice.

The invention relates to a pomacea canaliculata egg mass monitoring method based on a wireless remote control electric ship, which is characterized by comprising the following steps of: 1, manufacturing the wireless remote control electric ship; 2, fixing a camera; 3, connecting the camera with the mobile phone; 4, performing ampullaria gigas egg mass image acquisition: starting the electric ship and the camera, and acquiring the ampullaria gigas egg mass image; and 5, performing ampullaria gigas egg mass image analysis: importing the ampullaria gigas image data into a computer, opening Photoshop software to splice single ampullaria gigas egg mass images, and synthesizing an integral ampullaria gigas egg mass area image; and performing multi-point selection on the ampullaria gigas egg mass image by using a magic wand tool on the whole image of the ampullaria gigas egg mass area, outputting an ampullaria gigas egg mass distribution map, and observing the size and the number of the egg mass from the ampullaria gigas egg mass distribution map. The invention provides the pomacea canaliculata egg mass monitoring method based on the wireless remote control electric ship, and solves the problems of high risk and poor accuracy existing in manual onsite ocularization egg mass quantity and east-west estimation.

The invention discloses a metapenaeus affinis feed formula. The metapenaeus affinis feed is prepared from the following raw materials in parts by weight: 10-20 parts of corn starch, 10-15 parts of snail powder, 5-8 parts of peanut powder, 2-8 parts of calcium hydrophosphate, 1-5 parts of earthworm powder, 1-5 parts of rubus parvifolius, 1-5 parts of oldenlandia diffusa, 1-3 parts of zinc, 1-3 parts of iodine, 1-3 parts of vc, 3-8 parts of beer yeast, 12-15 parts of shell powder, 1-3 parts of seaweed meal, 5-8 parts of fish intestine powder, 5-8 parts of clam worm powder, 5-8 parts of freshwater shrimp powder, 10-12 parts of cassava meal, 0.1-0.4 part of table salt, 10-15 parts of ampullaria crossean powder, 20-25 parts of clamshell powder, 4-6 parts of fried soybean meal, 1-2 parts of rhizoma phragmitis, 1-2 parts of momordica grosvenori and 1-2 parts of lophatherum herb. Compared with the prior art, the metapenaeus affinis feed has the advantages that coarse food grains and extracts are combined, fire-clearing Chinese medicinal herbs are added, and a scientific mixture ratio is adopted, so that the growth and development demands of metapenaeus affinis are better met, the appetite is improved, the metapenaeus affinis death rate is lowered by 13 percent, and the yield is increased by 34 percent.

The invention discloses chicken feed. The chicken feed comprises, by weight, 50-60 parts of corn, 2-6 parts of sweet potatoes, 1-5 parts of pumpkins, 3-7 parts of rice, 5-10 parts of sweet potato seedling powder, 5-10 parts of peanut seedling powder, 2-6 parts of millet bran, 0.5-1.5 parts of limestone powder, 4-8 parts of ampullaria crossean, 3-9 parts of peanut shell powder, 5-10 parts of earthworms, 0.1-0.3 part of jujube powder, 0.5-1 part of lard oil, 0.5-1 part of calcium hydrophosphate, 0.3-0.5 part of sodium chloride, 1-3 parts of lysine, 0.5-1 part of methionine, 0.01-0.03 part of glycine, 0.2-0.4 part of cordyceps militaris, 0.1-0.3 part of wormwood, 0.02-0.04 part of talcum powder and 0.05-0.1 part of citric acid. The chicken feed has the advantages of being low in cost and good in quality.

The invention relates to a method for detecting pomacea canaliculata and controlling dosage by using an unmanned aerial vehicle. The method aims at monitoring and killing invading organisms, namely ampullaria gigas, in an agricultural rice field. The distribution of the ampullaria gigas in an area to be searched and killed is monitored through the unmanned aerial vehicle; the unmanned aerial vehicle can go deep into a farmland into which people cannot conveniently enter; the distribution density of the ampullaria gigas is speculated according to the number of the detected ampullaria gigas eggmasses, so that ampullaria gigas killing agents with different concentrations are selected, the purpose of accurate prevention and treatment is achieved, the prevention and treatment effect is achieved under the condition that the ampullaria gigas killing agents are used as little as possible, the cost is reduced, and the environment is protected.

The invention discloses chicken breeding feed. The chicken breeding feed comprises, by weight, 55-56 parts of corn, 2-4 parts of sweet potatoes, 2-5 parts of pumpkins, 5-7 parts of rice, 7-9 parts of sweet potato seedling powder, 5-8 parts of peanut seedling powder, 3-5 parts of millet bran, 0.7-1.1 parts of limestone powder, 2-7 parts of ampullaria crossean, 2-5 parts of peanut shell powder, 6-9 parts of earthworms, 5-8 parts of feather meal, 0.2-0.5 part of jujube powder, 0.5-0.7 part of lard oil, 0.5-0.7 part of calcium hydrophosphate, 0.2-0.6 part of sodium chloride, 1-3 parts of lysine, 0.5-0.9 part of methionine, 0.02-0.04 part of glycine, 0.3-0.6 part of cordyceps militaris, 0.1-0.4 part of wormwood, 0.01-0.04 part of talcum powder and 0.05-0.07 part of citric acid. The chicken breeding feed has the advantages of being low in cost and good in quality.

The invention relates to the technical field of alien invasive species control, and especially relates to application of 3-trifluoromethyl-4-nitrophenol in killing or preventing and controlling ampullaria gigas, a method for killing or preventing and controllingampullaria gigas, and a medicament. The invention provides application of 3-trifluoromethyl-4-nitrophenol in killing ampullaria gigas or preventing and controlling insect pests of the ampullaria gigas. The 3-trifluoromethyl-4-nitrophenol disclosed by the invention can be used for effectively preventing and controlling ampullaria gigas and is low in cost. A result of a specific embodiment of the invention shows that the 3-trifluoromethyl-4-nitrophenol has an obvious killing effect on the ampullaria gigas, and the prevention and controlling effect of the 3-trifluoromethyl-4-nitrophenol is equivalent to that of a common medicament niclosamide for killing the ampullaria gigas.

The invention relates to the technical field of pomacea canaliculata resourceful treatment and aquaculture, in particular to a macrobrachium rosenbergii breeding method based on pomacea canaliculata resourceful treatment. The method comprises the following steps: sequentially carrying out multi-stage purification on captured ampullaria gigas through temporary culture emptying, circulating micro-flowing water washing and EM bacterial liquid soaking, then integrally crushing the ampullaria gigas into ampullaria gigas meat paste, adding table salt and / or organic acid, and then mixing the ampullaria gigas meat paste with a vitamin-rich plant raw material and a vitamin mineral premix according to a ratio; and the soft pomacea canaliculata block bait which is not easy to foam and scatter is formed by extrusion or compression molding in cooperation with a hydrophilic gel binder. In a breeding pond of the macrobrachium rosenbergii, the soft block bait is matched with compound feed for the parent macrobrachium rosenbergii for precise feeding according to a limited proportion and a limited period of time, and the feeding amount and water change are dynamically adjusted in combination with water quality indexes, so that the gonad development and reproductive performance of the macrobrachium rosenbergii are improved, the water quality is stabilized, and the bait cost is reduced.

The invention discloses quick-acting fertilizer for bananas and belongs to the field of fertilizer. The quick-acting fertilizer for the bananas is prepared from the following raw materials in parts byweight: 22 to 30 parts of talcum powder, 10 to 15 parts of gypsum powder, 4 to 10 parts of diatomite, 5 to 9 parts of turf, 2 to 6 parts of ammonium dihydrogen phosphate powder, 2 to 6 parts of potassium carbonate powder, 11 to 16 parts of starch, 2 to 6 parts of potassium pyrophosphate, 2 to 7 parts of potassium metaphosphate, 1 to 4 parts of calcium hypochlorite, 1 to 4 parts of sodium hypochlorite, 1 to 4 parts of a coupling agent, 1 to 4 parts of a water-retaining agent, 30 to 35 parts of ammonium nitrate, 25 to 31 parts of potassium carbonate, 15 to 20 parts of calcium chloride, 4 to 8 parts of garlic, 4 to 8 parts of ampullaria crossean powder, 1 to 4 parts of microelement, 1 to 4 parts of nitrogen-fixing bacteria, 1 to 4 parts of rhizobium, 1 to 4 parts of phosphorus-dissolving bacteria, 3 to 8 parts of kaolin and 3 to 9 parts of diatomite. The fertilizer disclosed by the invention has high and stable fertilizer efficiency and balanced nutrients; the loss ratio of the fertilizer can be effectively reduced and the utilization efficiency of the fertilizer is improved; the quick and healthy growth of the bananas is promoted and the yield of the bananas is improved.

The invention discloses a pomacea canaliculata egg wrapping type inactivator and a preparation method thereof, and belongs to the technical field of biopesticides, the pomacea canaliculata egg wrapping type inactivator comprises a component A, a component B and a component C which are physically isolated and independently packaged, the component A is biopolymer matrix liquid and comprises a film-forming framework polymer; the film-forming skeleton polymer is alginate, a composite polymer, weak acid, an endogenous slow-release calcium source and water, and the composite polymer is chitosan; the component B is a gel activating solution and comprises a cross-linking activating agent, water and a modifying agent; the modifier is one of sodium citrate, cellulose nanocrystal or nanoclay; when the component A and the component B are in contact on the surface of an ampullaria gigas egg mass, the component A and the component B are subjected to an in-situ gel reaction to generate a layer of physical barrier gel film; the defects that an existing dehydrating agent scheme is not resistant to rain wash and poor in persistence are overcome, and the core component is derived from biomass and is environmentally friendly.

The invention discloses a GLUR6 gene related to low-temperature tolerance of pomacea canaliculata, an encoded protein and application of the GLUR6 gene, and belongs to the technical field of biology. The nucleotide sequence of the GLUR6 gene is as shown in SEQ ID NO: 1; the GLUR6 gene can be used as a target for pesticide screening and biological control of ampullaria gigas. The invention further provides dsRNA (double-stranded ribonucleic acid) of the GLUR6 gene. The green and environment-friendly biopesticide developed according to the dsRNA sequence can reduce the survival rate of ampullaria gigas in a low-temperature environment and the hatching rate of ampullaria gigas eggs. The method is rapid, effective and high in repeatability, and is an important supplement to the GLUR6 gene family. The low-temperature tolerance of the ampullaria gigas is reduced by blocking the expression of the GLUR6 gene in the ampullaria gigas, so that the purposes of controlling overwintering of the ampullaria gigas in soil in winter, reducing propagation of the ampullaria gigas and continuing to spread to the north are achieved. The method disclosed by the invention has important significance in research and determination of biology, ecology and novel insecticides of the ampullaria gigas and mechanism research of the ampullaria gigas on low-temperature tolerance.

The utility model relates to the technical field of pomacea canaliculata trapping, and discloses a pomacea canaliculata trapper which comprises a collecting box, the upper end of the collecting box is fixedly connected with a shielding mechanism through a plurality of supporting rods, and one side of the collecting box and one side of the shielding mechanism are jointly provided with a fixing rod. The shielding mechanism comprises a supporting plate and a soft nylon net bag installed at the lower end of the supporting plate, and a balancing weight is arranged at the lower end of the inner side of the soft nylon net bag. The fixing rod is inserted into sludge, so that the whole trapper can be fixed, the situation that the trapper cannot be found after use is avoided, and the trapper can be recycled and reused easily; according to the pomacea canaliculata collecting box, the soft nylon net bag covers the inlet in the collecting box, when the pomacea canaliculata crawl towards the inlet, the soft nylon net bag can be easily jacked open from the outside, the soft nylon net bag is attached to the inlet, and the pomacea canaliculata cannot crawl out from the interior of the box body; and external garbage and sundries can be prevented from falling into the box body from the inlet.

The invention discloses a physical and biological cooperative prevention and control effect evaluation method based on ampullaria gigas, and the method comprises the steps: collecting experiment monitoring data and associated data of a preset ampullaria gigas region, and carrying out the preprocessing of the experiment monitoring data and the associated data; performing snail control and measure persistence analysis according to the biological control data to obtain a natural enemy satiation threshold value and a resistance gene frequency, correcting the natural enemy satiation threshold value by adopting a predator and prey dynamic model, and correcting the resistance gene frequency by adopting selective pressure; intercepting and trapping analysis is carried out according to the physical prevention and control data to obtain interception efficiency, the interception efficiency is adjusted by adopting snail behavior response and temperature and humidity coupling, and treatment efficiency data is obtained according to the cooperative prevention and control data; and according to the natural enemy satiation threshold value, the resistance gene frequency, the interception efficiency and the treatment efficiency data, constructing a three-dimensional pomacea canaliculata physical and biological cooperative control effect evaluation model, and outputting an evaluation result.

The invention discloses metaldehyde granules with attractive property and waterproofness and a preparation method of the metaldehyde granules. The preparation method comprises the following steps: adding water into metaldehyde, a filler, a filler attractant and an adhesive, mixing to prepare particles, carrying out spray coating on the prepared particles by using a coating agent containing polyethylene glycol monomethacrylate or sodium lignin sulfonate, and drying to prepare the metaldehyde granule with both attractive property and water resistance. Tests show that the metaldehyde granule has strong attraction to the ampullaria gigas, metaldehyde in the granule cannot be quickly separated out into a water body after the metaldehyde granule encounters water, the killing effect of the metaldehyde on the ampullaria gigas can be exerted for a long time, and efficient prevention and treatment of the ampullaria gigas are facilitated.

The invention discloses a TPR1 gene related to low-temperature tolerance of pomacea canaliculata, an encoded protein and application of the TPR1 gene, and belongs to the technical field of biology. The method is rapid, effective and high in repeatability, and is an important supplement to the TPR1 gene family. The pomacea canaliculata is an important invasive organism, development of novel biopesticides is facilitated, and the low-temperature tolerance of the pomacea canaliculata is reduced by blocking expression of the TPR1 gene in the pomacea canaliculata, so that the purpose of controlling further north invasion of the pomacea canaliculata is achieved. Meanwhile, by interfering the expression of the TPR1 gene, the hatching rate of the eggs of the ampullaria gigas can be remarkably reduced, the hatching duration of the eggs can be prolonged, and therefore the harm of invasion of the ampullaria gigas is reduced. The method is of great significance to research and determination of biology, ecology and novel insecticides of the ampullaria gigas and research on a resistance mechanism of the ampullaria gigas to low-temperature tolerance.

The invention discloses a pomacea canaliculata environment DNA specificity detection line and a biomass evaluation method, the technical scheme is as follows: the pomacea canaliculata environment DNA specificity detection line comprises an upstream primer, a downstream primer and a probe, and the method uses the pomacea canaliculata environment DNA specificity detection line to detect the pomacea canaliculata environment DNA concentration and obtain the environment DNA detection quantity; and calculating the biomass of the pomacea canaliculata according to the obtained environmental DNA detection quantity, namely, detecting the environmental DNA concentration of the pomacea canaliculata, and calculating the biomass of the pomacea canaliculata according to the environmental DNA detection quantity by using a pomacea canaliculata biomass calculation formula. The method has the advantages that only the pomacea canaliculata is specifically detected, other freshwater snails cannot be detected wrongly, the problem of wrong detection is effectively solved, particularly, different haplotypes of various pomacea canaliculata are covered, the environmental DNA monitoring work efficiency of the pomacea canaliculata is improved, and the established biomass evaluation model based on environmental DNA degradation and shedding rate has the advantages that the environmental DNA degradation and shedding rate of the pomacea canaliculata can be effectively evaluated. The method can be successfully applied to field biomass evaluation of ampullaria gigas.