The invention relates to an aerobic culture and gas collection integrated indoor soil culture apparatus, and an application thereof. The apparatus comprises a culture container, the top mouth of the culture container is provided with a separator plate matched with the top mouth, the separator plate comprises a separator plate body and a gas opening and closing device, the separator plate is in closed fixed connection with an aeration container with an open top end, the sidewall of the aeration container is provided with an aeration opening, the ventilation container is covered with a top cover, the top cover is provided with a gas inlet hole and a gas collecting hole, a gas inlet tube is fixed in the gas inlet hole, one end of the gas inlet tube is inserted into the culture container, the other end of the gas inlet tube is connected with a balancing tube, a gas collecting tube is fixed in the gas collecting hole, one end of the gas collecting tube is inserted into the culture container, and the other end of the gas collecting tube is connected with a gas collecting needle. The apparatus has the advantages of implementation of aerobic culture and gas collection on the apparatus, small size, use in a limited space, convenience in operation, realization of closed culture under normal pressure, quantification of the initial culture concentration of a target gas, solving of the problem of target gas absorption due to the negative pressure caused by sampling, and real and effective reality-approaching culture and detection result.