The invention discloses a micro-fluidic chip flow velocity sensor based on micro-nano optical fibers. The micro-fluidic chip flow velocity sensor comprises a micro-fluidic chip, micro-nano optical fibers and a flexible thin film, the thickness of the flexible thin film is smaller than or equal to 100 microns, a refractive index of the flexible thin film is smaller than that of the micro-nano optical fiber, the flexible film coats a fluid channel of the micro-fluidic chip, the flexible film is provided with openings at an inlet and an outlet of the fluid channel respectively, a stretching partof the micro-nano optical fiber stretches across the fluid channel at the to-be-measured position of the fluid channel, and the stretching part of the micro-nano optical fiber, a tapering transition area and non-stretching parts at the two ends are partially embedded in the flexible film. When fluid in the fluid channel flows, the flexible film is bent outwards under pressure, and due to the increase of the bending loss of the micro-nano optical fiber, an output light intensity signal is reduced accordingly, so that flow velocity sensing is realized. According to the invention, multi-point flow velocity detection can be realized, flow velocity detection can be carried out on an existing micro-fluidic chip, and high-sensitivity flow velocity sensing is realized.