The invention discloses a preparation method of 1,4-diallyl isoquinoline synthesized by using a palladium catalyst. The structural formula of 1,4-diallyl isoquinoline is shown in the specification, wherein R1 refers to one or more substituents connected to a benzene ring, and is selected from one of H, chlorine and -O-CH2-O-, and R2 is one of phenyl, p-methoxyphenyl, and 1-cyclohexenyl. In the process of preparing, 1-methyl azide-2-ethinyl-benzene shown in the specification is taken as a raw material, and reacted with allyl methyl carbonate in the presence of a Pd(PPh3)4 catalyst, an alkaline cocatalyst and an organic solvent, so that 1,4-diallyl isoquinoline is obtained. According to the invention, a 1,4-diallyl isoquinoline compound can be efficiently synthesized by using a one-pot method; the preparation method is high in conversion efficiency, short in reaction time and neutral in reaction conditions, and has no generated by-product; the method is low in raw material cost, simple in operation, low in pollution to the environment, and beneficial to industrialized production; and products are widely used, and can be used as intermediates for further synthesizing more complex compounds.