The invention discloses a positioning structure for an AGV assembly vehicle tray. The structure comprises a supporting mechanism, a jacking mechanism, a floating mechanism and a positioning mechanism.The supporting mechanism is used for bearing components, the jacking mechanism comprises a jacking air cylinder, a guide rod, two bearings, two sliding blocks and two sliding grooves, the sliding grooves comprises obliquely-upward sliding groove slopes, the floating mechanism comprises a third beam, the third beam is fixedly connected with the tops of the two sliding blocks, and the positioning mechanism comprises two positioning air cylinders located at the two ends of the third beam and two positioning pins connected with piston rods of the two positioning air cylinders correspondingly. According to the structure, the jacking mechanism is stable in operation, vertical space is saved, push force far larger than air cylinder force can be output, falling is prevented, the floating mechanism can ensure horizontal suspension of the tray, the positioning mechanism is high in positioning precision, and noise is low.