32results about How to "Uniform reaction conditions" patented technology

The invention relates to a quick and efficient non-template agent hydro-thermal synthesizing method. The system can synthesize an alpha-molybdenum trioxide nanometer rod and a high-density echinoid molybdenum oxide based nanometer materials. Molybdenum peroxide acid prepared from molybdenum trioxide and aqueous hydrogen peroxide solution is used as a precursor, is produced into scattered alpha-molybdenum trioxide nanometer rod by hydro-thermal synthesis at a temperature of between 80 and 180 DEG C, and is produced into the peroxide modified molybdenum oxide hydrate by hydro-thermal synthesis at a temperature of between 65 and 75 DEG C. The hydrate is a multiscale structure; a nanometer thin slice, a micron prism and a nanometer rod-shaped structure unit are divergently assembled into a micron-size high-density echinoid structure. The hydrate is roasted to obtain high-density echinoid alpha-molybdenum trioxide. Modulation of the synthesizing condition can realize fine adjustment for appearance of the nanometer rod, the micron-size echinoid structure and the structure unit thereof. The method uses raw materials with low cost, has the advantages of simple technical process, controllable conditions and the like, and can promote research and application of the molybdenum oxide in the fields of sensors, field transmission, electrode materials and so on.

The invention belongs to the technical field of biological reagents, and relates to the assay of gene rearrangement clonality related to lymphoid neoplasm diagnosis. The development of a full-primer coverage, low-cost and easy-to-operate lymphoid tissue gene rearrangement assay kit capable of being popularized in ordinary hospitals is urgently needed clinically. The invention discloses a lymphocyte gene rearrangement clonality assay kit, which is all-liquid reagent based on combined primers, and the invention also provides a related assay method and a hierarchical optimization scheme. When the kit is used for assaying the clonality of lymphocyte gene rearrangement, the assay accuracy can reach more than 99 percent, moreover, the steps of the assay method are simple, the total cost is low, and the invention is highly worth popularizing clinically.

The invention relates to a detecting deice, and in particular relates to a multi-index detecting device, a kit and an application thereof. When a plurality of indexes of a sample are detected in the prior art, detection reagents for different indexes are needed to use, and the samples are respectively added to reaction containers of the agents, so that the operation is very inconvenient. For solving the defect, the invention provides an individual multi-index detecting device. The detecting device comprises at least one multi-hole detecting strip, wherein at least two detecting holes are formed on the multi-hole detecting strip; each of the detecting holes comprises a lower part, a bottom part and an upper part; the bottom parts of the detecting holes are closed, the lower parts of the detecting holes are independent with each other; the lower parts and the bottom parts of different holes can be used for simultaneously covering different bioactive substances; and the upper parts of the detecting holes are communicated with each other. The multi-index detecting device provided by the invention can add the samples to a plurality of detecting holes by one-step sampling, so that a plurality of indexes of the samples are detected, and the operation is convenient and quick.

The invention discloses a method for preparing anti-UV fabric by an in-situ method, belonging to the technical field of fabric post treatment. The method includes the steps of: weighing Ce (NO3)3.6H20, dissolving the Ce (NO3)3.6H20 in water, stirring to enable the Ce(NO3)3.6H20 to dissolve evenly so as to prepare Ce(NO3)3 solution with concentration of 1-5g/L; and adding a surfactant, aqueous ammonia and a strong oxidizer into the Ce(NO3)3 solution sequentially, stirring to be even, soaking the fabric in the solution for 10-120mins, taking out the fabric, and drying for 1-5mins at 80-180 DEG C. In this way, washable anti-UV fabric can be prepared. The weight ratio of the Ce (NO3)3.6H20, the surfactant, the aqueous ammonia and the hydrogen peroxide is 10:5-24:1-9:1-5; and the surfactant iscetyl-trimethyl ammonium bromide or dodecyl benzene sulfonic acid sodium salt. The method is simple in process and low in cost, and through the method, anti-UV fabric which can be tightly bonded withnanometer material can be prepared.

The invention discloses a detection probe, a liquid phase chip and a detection method thereof for EGFR gene mutation sites. The EGFR gene mutation detection liquid phase chip mainly comprises a microsphere for wrapping the probe; and a primer used for being amplified with a target sequence with 19 exons and/or 21 exons. By adopting the EGFR gene mutation detection liquid phase chip and the detection method, the gene mutation sites with higher relative frequency can be detected simultaneously, the 19 exons and the 21 exons can be detected separately, and also can be detected simultaneously, the reaction conditions during the detection are uniform, the specificity of the detected result is good, the sensitivity is high, the detection accuracy reaches above 90 percent, and the detection timeis short. The detection method not only can detect the EGFR gene mutation in the tumor tissue sample, but also can detect the EGFR gene mutation in the body fluid of the patient with tumor and also can perform dynamic detection.

The invention discloses a method for batched formation of square beams for biological soil reinforcement. The method for batched formation of the square beams for biological soil reinforcement comprises the following steps that (1) materials required by a mold are prepared; (2) cut organic glass plates provided with holes are assembled; (3) a light mold required for tailoring of geotechnical cloth is manufactured, and the geotechnical cloth is tailored according to the sizes of light materials; (4) the geotechnical cloth is placed in square beam mold cavity units, and sandy soil is added into cavities defined by the geotechnical cloth; (5) prepared bacteria are poured into test piece molds; (6) the two or four wetted test piece molds are placed in a container side by side, and a plurality of layers of mold sets are arranged according to the actual condition; (7) the multiple layers of mold sets are soaked in a chemical solution; and (8) the square beams for biological soil reinforcement are formed after a reaction. According to the method for batched formation of the square beams for biological soil reinforcement, by means of the flatness of the surface of organic glass and the good water permeability of the holes, cuboid test pieces formed after the chemical reaction are angular, high in surface flatness, uniform in texture and stable in mechanical property, and a scientific research test for testing the bending performance can be conducted in the later stage conveniently.

An active portion of a nano-size element is selectively destroyed by applying an electric potential to the nano-size element having a carbon network structure of carbon as an outer shell while immersed in an electrolytic solution. For example, an electric potential of 1.20 to 1.60 V (RHE) is applied to the carbon nanotubes in electrolytic solution so as to oxidize the active portion in each carbon nanotube and to thereby selectively remove the ends. Consequently, an open end is created in the carbon nanotube.

The invention provides an industrial sewage treatment method, which comprises the following steps of (1) mechanical treatment; (2) softening treatment; (3) reinforced coagulation; (4) pulse discharging plasma-photocatalysis cooperated degradation of organic matters; (4) ultrasound cooperated nanometer zero-valent iron treatment; (6) iron wire cage framework cellulose aerogel adsorption; (7) ultrafiltration/nanofiltration-reverse osmosis integrated film method treatment. The industrial sewage treatment method provided by the invention has the advantages that the process is simple; the operationperiod is short; contaminants such as suspended matters, organic matters and salts contained in the industrial sewage can be thoroughly removed; a good removal effect can be achieved on various kindsof continuous organic contaminants and heavy metal; in addition, the commonality is realized on the contaminant removal; the heavy metal recovery rate is high; the economic benefits are good; the process flexibility is high; the anti-impact load capability is high; the sewage recovery rate is high; the method is applicable to the sewage treatment in various fields of electric power, photovoltaics, photoelectricity, semi-conductors, electrics, pharmacy, metallurgy, petrochemical engineering and the like.

The invention discloses a probe for detecting EGFR gene exon 20 mutation, a liquidoid chip and a detection method thereof. The liquidoid chip of the EGFR gene exon 20 mutation comprises a microsphere enveloped by the probe, the microsphere enveloped by a wild amido modification probe with base sequence selected from any of SEQ ID NO: 1-SEQ ID NO: 6 aiming at exon 20 and the microsphere enveloped by a mutant amido modification probe with the base sequence selected from any of SEQ ID NO: 7-SEQ ID NO: 12 aiming at exon 20. A space arm is used for connecting the base sequence and the amido of each probe. Every microsphere has different color codes. The liquidoid chip of the EGFR gene exon 20 mutation also comprises a primer used for amplifying a target sequence with exon 20 mutant site. The method provided by the invention is fast and exact, is simple for operating and improves the detection efficiency greatly.

The invention discloses a device and method for large-scale continuous preparation of metal nanoparticles, and belongs to the technical field of preparation of metal nanoparticles. The device comprises a micro-flow injection pump, micro-flow channels, a Y-shaped three-way pipe, a heating plate system for reaction temperature control and a temperature control product collection pool. The channels are parallel and straight, the structure is flat and straight, the solvent circulation resistance is small, and the defect that traditional micro-flow channels are blocked is overcome; the applicability to a platform is strong, and various solvents and various metals can react; the reaction conditions are uniform; and segmented heating is achieved, multi-point accurate regulation and control are achieved, and the large-scale production capacity is achieved.

The invention belongs to the technical field of nano materials, and discloses a simple preparation method of an Ag-Au composite nano periodic structure with high SERS activity. The simple preparationmethod comprises the following steps that a layer of silver film is evaporated on the surface of a single-pass anodic aluminum oxide template pore channel, and the silver film forms silver nanoparticles which are uniformly distributed in the pore channel through a high-temperature confinement spheroidizing process; and a substitution reaction is carried out by using Au<1> solution to obtain the Ag-Au composite nano periodic structure for SERS activity detection of various probe molecules. Compared with the prior art, the method has the advantages that the problem of poor repeatability of SERSsignals of traditional nano-particle colloid is solved, controllable preparation as well as SERS high-repeatability and high-activity detection of the large-area Ag-Au composite nano periodic structure are realized, the preparation cost of the periodic nano structure is greatly reduced, and the method is suitable for batch production.

The invention discloses a method, primers and a kit for detecting PD-L1 gene expression quantity through real-time fluorescent quantitative PCR. The method comprises the following steps: (1) synchronously extracting DNA and mRNA in a sample; (2) synthesizing a first chain cDNA by taking the to-be-detected mRNA as a template through a special reverse transcription primer; and (3) carrying out dual-channel real-time fluorescent quantitative PCR amplification by respectively adopting a primer corresponding to the specific sequence on the reverse transcription primer and a specific primer corresponding to a reference gene and taking a reverse transcription product cDNA and reference gene DNA as templates while amplifying a target gene cDNA and a reference gene, and calculating the copy numberratio of the target gene to the reference gene in the sample according to the respective standard curves by taking a plasmid constructed by linking the reference gene and the target gene sequence as astandard substance to obtain the expression level of the target gene, so that various experimental operation errors are synchronously corrected, and the reliability and the repeatability of the result are ensured.