32results about How to "Uniform reaction conditions" patented technology

The invention relates to a detecting deice, and in particular relates to a multi-index detecting device, a kit and an application thereof. When a plurality of indexes of a sample are detected in the prior art, detection reagents for different indexes are needed to use, and the samples are respectively added to reaction containers of the agents, so that the operation is very inconvenient. For solving the defect, the invention provides an individual multi-index detecting device. The detecting device comprises at least one multi-hole detecting strip, wherein at least two detecting holes are formed on the multi-hole detecting strip; each of the detecting holes comprises a lower part, a bottom part and an upper part; the bottom parts of the detecting holes are closed, the lower parts of the detecting holes are independent with each other; the lower parts and the bottom parts of different holes can be used for simultaneously covering different bioactive substances; and the upper parts of the detecting holes are communicated with each other. The multi-index detecting device provided by the invention can add the samples to a plurality of detecting holes by one-step sampling, so that a plurality of indexes of the samples are detected, and the operation is convenient and quick.

The current is directed to a device comprising, a reaction chamber where a reaction takes place, one or more feeding chambers which contain feeding solution comprising chemicals required for the reaction, and one or more porous membranes separating the reaction chamber from the one or more feeding chambers, and wherein the one or more porous membranes are in substantially vertical position. The current invention is also directed to an apparatus comprising a plurality of devices, wherein each device comprises of a reaction chamber where a reaction takes place, one or more feeding chambers which contain feeding solution comprising chemicals required for the reaction, and one or more porous membranes which separate the reaction chamber from feeding chambers, wherein the one or more porous membranes are in substantially vertical position. These devices and apparatuses can be used for high throughput synthesis of biomolecules and chemicals and biological screening assays.

The invention discloses a method for preparing anti-UV fabric by an in-situ method, belonging to the technical field of fabric post treatment. The method includes the steps of: weighing Ce (NO3)3.6H20, dissolving the Ce (NO3)3.6H20 in water, stirring to enable the Ce(NO3)3.6H20 to dissolve evenly so as to prepare Ce(NO3)3 solution with concentration of 1-5g / L; and adding a surfactant, aqueous ammonia and a strong oxidizer into the Ce(NO3)3 solution sequentially, stirring to be even, soaking the fabric in the solution for 10-120mins, taking out the fabric, and drying for 1-5mins at 80-180 DEG C. In this way, washable anti-UV fabric can be prepared. The weight ratio of the Ce (NO3)3.6H20, the surfactant, the aqueous ammonia and the hydrogen peroxide is 10:5-24:1-9:1-5; and the surfactant iscetyl-trimethyl ammonium bromide or dodecyl benzene sulfonic acid sodium salt. The method is simple in process and low in cost, and through the method, anti-UV fabric which can be tightly bonded withnanometer material can be prepared.

The invention discloses a probe for detecting EGFR gene exon 20 mutation, a liquidoid chip and a detection method thereof. The liquidoid chip of the EGFR gene exon 20 mutation comprises a microsphere enveloped by the probe, the microsphere enveloped by a wild amido modification probe with base sequence selected from any of SEQ ID NO: 1-SEQ ID NO: 6 aiming at exon 20 and the microsphere enveloped by a mutant amido modification probe with the base sequence selected from any of SEQ ID NO: 7-SEQ ID NO: 12 aiming at exon 20. A space arm is used for connecting the base sequence and the amido of each probe. Every microsphere has different color codes. The liquidoid chip of the EGFR gene exon 20 mutation also comprises a primer used for amplifying a target sequence with exon 20 mutant site. The method provided by the invention is fast and exact, is simple for operating and improves the detection efficiency greatly.

Probes, liquidchip-based products, and methods for detection of EGFR gene mutation are disclosed. The liquid chip for detection of EGFR gene mutation mainly includes: microspheres coated with probes; primers for amplifying target sequence in exon 19 and / or exon 21.The liquid chip and methods can detect mutations with relatively higher frequency in EGFR gene, and detect mutations in exon 19 and 21 simultaneously or separately. The assay has high specificity and sensitivity with uniform reaction condition. Detection has over 90 percent accuracy and takes less time. The method can be applied not only to detection of EGFR gene mutations in tumor tissue samples but also to those in humour samples from cancer patients. Thus it can monitor dynamicly.

The invention relates to a detecting deice, and in particular relates to a multi-index detecting device, a kit and an application thereof. When a plurality of indexes of a sample are detected in the prior art, detection reagents for different indexes are needed to use, and the samples are respectively added to reaction containers of the agents, so that the operation is very inconvenient. For solving the defect, the invention provides an individual multi-index detecting device. The detecting device comprises at least one multi-hole detecting strip, wherein at least two detecting holes are formed on the multi-hole detecting strip; each of the detecting holes comprises a lower part, a bottom part and an upper part; the bottom parts of the detecting holes are closed, the lower parts of the detecting holes are independent with each other; the lower parts and the bottom parts of different holes can be used for simultaneously covering different bioactive substances; and the upper parts of the detecting holes are communicated with each other. The multi-index detecting device provided by the invention can add the samples to a plurality of detecting holes by one-step sampling, so that a plurality of indexes of the samples are detected, and the operation is convenient and quick.