The invention relates to a preparation method of a wet tissue for improving eyesight, and belongs to the field of traditional Chinese medicine health care. Aiming at the problems that eye drops directly act on the eyeball, and when the liquid directly contacts the eyeball, it will cause mucous membrane irritation and pain, eyelid skin flushing, and conjunctival edema. The invention provides a preparation method of a wet tissue for improving eyesight. The present invention adopts medicinal materials such as chamomile flower, frost mulberry leaf, mint, antelope horn tip, habitat, and Prunella vulgaris to eliminate tension, relieve eye fatigue, clear liver and improve eyesight, and relieve liver fire. above, prepared as wet wipes, the wet wipes prepared by the present invention wipe the eyes, or apply on the eyelids, do not directly contact with the eyeballs, avoid causing irritation to the eyeballs, the wet wipes prepared by the present invention can increase eye metabolism, promote blood circulation Circulation is very beneficial to eye health.