The invention relates to a cattle killing machine, and belongs to slaughtering equipment. The cattle killing machine comprises a prisoner tube mechanism, a front push plate, a rear push plate, a cattle rump drive mechanism, a stranglehold mechanism, a cattle head turnover mechanism, a post-slaughter cattle ejecting mechanism, a cavity door start-stop mechanism and a power drive mechanism, wherein the prisoner tube mechanism comprises first and second bases, first and second beams, a top plate, a bottom plate, left and right rotary disks and a cattle containing groove; one end of the lower part of the front push plate is arranged on the left rotary disk, and the other end of the lower part of the front push plate is arranged on the right rotary disk; one end of the lower part of the rear push plate is connected with the left rotary disk, and the other end of the lower part of the rear push plate is connected with the right rotary disk; the cattle rump drive mechanism is arranged on a wall body on one side of the front push plate, and the stranglehold mechanism is arranged on the center position of the first base; the cattle head turnover mechanism is arranged on the stranglehold mechanism, and the post-slaughter cattle ejecting mechanism is arranged on the upper part of one side of the front push plate; the cavity door start-stop mechanism is arranged on the right rotary disk; and the power drive mechanism is arranged one side of the base, the stranglehold mechanism comprises a stranglehold gate action cylinder and a stranglehold door, and the cattle head turnover mechanism comprises a head turning action cylinder, a head turning frame, a connecting rod and a rocker arm. The prisoner tube mechanism is avoided being damaged, the balance property of a neck clamping force is good, and an oppressing effect of a laryngeal of cattle is improved.