The invention discloses a preparation method of an efficient biocontrol growth-promoting ginseng composite microbial fertilizer, relates to the technical field of ecological planting of ginseng, and solves the problem that the yield of ginseng is increased mainly by chemical fertilizers, pesticides and the like in existing non-forest land ginseng cultivation. The preparation method comprises the following steps: I, collecting; 2, screening and separating; and 3, preparing the compound microbial fertilizer. The method has the advantages that: 1, the four strains have strong stress resistance and multiplication capacity and promote growth mutually; 2, the quantity of viable bacteria is large and can reach 16.67 * 10 < 9 > CFU * ml < -1 >; 3, the fertilizer efficiency is activated, the reutilization of nitrogen, phosphorus and potassium is increased, the absorption of ginseng roots is promoted, the fertilizer has slow release property, persistence and long fertilizer efficiency, and the yield and quality of ginseng are improved; 4, the structural characteristics of soil microbial florae are improved, the diversity of effective microbial florae is increased, and the biocontrol function is achieved; and 5, the method is simple to operate, low in cost, remarkable in effect, environment-friendly and the like. The method is mainly used for ecological planting biocontrol, fertilizer efficiency increase and yield increase of ginseng.