The invention discloses a microalgae 
biodiesel production method. The production method comprises the following steps of inoculating 
nannochloropsis oculata to a culture medium, irradiating through a red-blue dual-color LED light supplementing lamp for 8 to 14 hours per day, cultivating for 6 to 8 days at a temperature of 24 to 26 DEG C, and collecting 
nannochloropsis oculata cells; performing a wall breaking process on the collected 
nannochloropsis oculata cells through ultrasonic wave, then extracting through an 
organic solvent to obtain microalgae 
grease, and then adding the microalgae 
grease, methyl 
alcohol and a 
zeolite molecular sieve catalyst into to a reactor according to a 
mass ratio of 1 : (5 to 7) : (0.01 to 0.03) to perform 
esterification reaction, so as to obtain an esterification product; then separating and purifying the esterification product to obtain the 
biodiesel. According to the production method, the 
nannochloropsis oculata which is short in production period, wide in distribution, strong in 
photosynthesis and high in 
grease content is utilized as a 
raw material, so that the problem of 
vegetable oil and fat raw materials in 
biodiesel development is solved. The production method also has the advantages of being simple in preparation technology, high in 
catalytic efficiency, high in grease extraction rate, high in product yield, and the like, and also lays a good foundation for industrialization and scale of the biodiesel.