The invention discloses an endoscope sterilizer. The endoscope sterilizer comprises a cabinet body, a primary washing bin, a primary washing bin cover, a top cover spraying head, a cleaning pipe, endoscope fixing frames, a drying bin, a drying bin cover, a top cover drying head, a drying exhaust port, a drainage chamber, a basin cover, a cleaning basin, an inner cavity cleaning head, an enzyme washing liquid metering pump, a disinfectant infusion pump, an ultrasonic generator, ultrasonic vibrators, an enzyme washing liquid storage tank, a disinfectant storage tank, a high-pressure water pump,a controller, a high-pressure air pump, a water inlet pipe, an air inlet pipe, a water distribution valve, an air distribution valve, inner cavity water delivering seats, inner cavity gas delivering seats, cleaning tank drainage valves and drainage pipes. According to the endoscope sterilizer, the disinfection effect is improved, the whole process is automatically mechanical cleaned, labor is reduced, the mechanical structure is compact, the space is saved, the functioned and the staged cleaning can be realized through the controller, the cleaning basin is compact, the use amount of the cleaning agent is reduced, and the disinfection cost is greatly reduced.