The invention discloses a method for extracting 
Botryosphaeria dothidea 
hypha RNA (ribonucleic acid). The method comprises the following steps: 
grinding Botryosphaeria dothidea hyphae preserved in 
liquid nitrogen into 
powder in the 
liquid nitrogen; transferring the 
powder into a precooled 
centrifuge tube, adding 1mL of 
Trizol leaching liquor to every 90-110mg of 
powder, uniformly mixing by shaking, and standing at 
room temperature; adding 
chloroform, oscillating intensely, standing at 
room temperature, and centrifugating; putting the supernate into a new 
centrifuge tube, adding isopropanol and a precipitate 
buffer solution, and uniformly mixing in a reversed mode; centrifugating; discarding the supernate, adding 1mL of a 75 vol% 
ethanol solution to clean the 
RNA precipitate block, centrifugating, and discarding the supernate; repeating the cleaning operation; and discarding the 
ethanol, opening the cap, standing at 
room temperature for 10-15 minutes or carrying out 
vacuum drying to completely volatilize the 
ethanol, thereby obtaining the precipitate 
RNA product. The RNA extracted by the method has the advantages of high purity and high yield.