The invention discloses a method for extracting
Botryosphaeria dothidea
hypha RNA (ribonucleic acid). The method comprises the following steps:
grinding Botryosphaeria dothidea hyphae preserved in
liquid nitrogen into
powder in the
liquid nitrogen; transferring the
powder into a precooled
centrifuge tube, adding 1mL of
Trizol leaching liquor to every 90-110mg of
powder, uniformly mixing by shaking, and standing at
room temperature; adding
chloroform, oscillating intensely, standing at
room temperature, and centrifugating; putting the supernate into a new
centrifuge tube, adding isopropanol and a precipitate
buffer solution, and uniformly mixing in a reversed mode; centrifugating; discarding the supernate, adding 1mL of a 75 vol%
ethanol solution to clean the
RNA precipitate block, centrifugating, and discarding the supernate; repeating the cleaning operation; and discarding the
ethanol, opening the cap, standing at
room temperature for 10-15 minutes or carrying out
vacuum drying to completely volatilize the
ethanol, thereby obtaining the precipitate
RNA product. The RNA extracted by the method has the advantages of high purity and high yield.