The invention belongs to the technical field of composite materials, and particularly discloses a bio-optical composite material, a preparation method and an application thereof. The bio-optical composite material comprises the following raw materials in parts by weight: 0.5-3 parts of mica, 0.5-2 parts of mirabilite, 0.5-3 parts of haematite, 0.5-1 part of gypsum, 0.5-1 part of magnetite, 0.5-3 parts of cinnabar, 0.5-2 parts of tourmaline, 0.5-3 parts of alum and 0.5-2 parts of medical stone. The raw material fine powder of the bio-optical composite material is evenly mixed in a high-speed mixer, and is irradiated for 12-24 hours by a laser with 457-600nm frequency band and a continuous output power of 400mW. The composite material can convert infrared waves emitted by human bodies to generate short-wave biological waves, can promote cell metabolism, improve cell pathology and improve cell physiology, has the functions of quickly relieving pain, repairing cell damage, dispelling glandhyperplasia, improving cell hypoxia, enhancing muscle fiber strength, strengthening physiological state of human organs and the like, can be used for medical treatment and human body rehabilitation health care, such as preparing multiple medical patches, eyeglass frames and other human body contact articles for daily use.