The invention provides a nasal spray and a preparation method thereof. The nasal spray is prepared from sea salt, glycerin, sodium hyaluronate, ethylparaben, ethanol, potassium sorbate, a eucalyptus globulus leaf extracting solution, a bay leaf extracting solution, a sandalwood extracting solution and purified water through special processes and flows. The types of sterilizing agents in the nasalspray are changed, and natural sterilizing and inflammation-diminishing substances in plants of eucalyptus globulus leaves, bay leaves and sandalwood are adopted instead of the conventional antibioticmedicaments such as aspirin, ibuprofen and chlortrimeton, so that the side effects on human body are reduced, and bacteria in the nasal cavity are prevented from developing drug tolerance. The glycerin and the sodium hyaluronate are adopted for forming a layer of moisturizing film on the inner wall of the nasal cavity, so that the durability of the product in nasal cavity cleaning and moisteningis improved on the premise of removing pathogenic bacteria in the nasal cavity, and the nasal cavity can keep clean and wet for a long time.