54 results about "Neuroscience research" patented technology

Apparatus and methods in which very small volumes of material may be extracted, delivered, interrogated or stimulated via optical, electromagnetic or mechanical means, in vivo or in vitro, for site-specific detection, characterization, stimulation, diagnostics or therapy, comprising optical, fluidic, chemical, electromagnetic and biological techniques applied via a microprobe in a single intra-parenchymal tissue perforation procedure in the brain. The primary use of the device is in neuroscience research, clinical diagnostics and therapeutics applications in the brain, however, the device may also be beneficially applied to other organs and biological systems. Human clinical applications may include neurosurgical intra-operative monitoring, extra-operative chronic monitoring of devices introduced in an operation, and diagnostic monitoring combined with simultaneous neuroimaging.

The invention discloses a psychological assessment system. The system comprises at least one mobile terminal client, at least one brain wave testing instrument and a data server, wherein the mobile terminal client is used for collecting psychological assessment data of subjects and displaying a psychological assessment result to the subjects; the brain wave testing instrument is used for monitoring brain wave data of the subjects and transmitting the brain wave data to the mobile terminal client; the brain wave testing instruments and the mobile terminal clients are the same in number and are in one-to-one correspondence in a wireless manner; and the data server is used for obtaining the psychological assessment data and the brain wave data from the mobile terminal client, obtaining the psychological assessment result through statistical processing and analysis and transmitting the psychological assessment result to the mobile terminal client. The psychological assessment system disclosed by the invention can be applied to criminal investigation, neuroscience research, psychotherapy and so on and can be also applied to psychological investigation of population within a large range; the accuracy and effectiveness of the psychological assessment can be effectively enhanced; and information processing efficiency is increased.

The invention discloses a light control and neural information detection system. The system comprises a light stimulation control module, an electrochemical detection module, an electrophysiological detection module, a synchronous data acquisition module, a main control module and a synchronous control module, wherein the light stimulation control module can be combined with the optogenetics technology to carry out light control via laser fibers or LED lamp electrodes; the electrochemical detection module is used for detecting neurochemical signals; the electrophysiological detection module is connected with a microelectrode array and is used for detecting neural electrophysiological signals; the synchronous data acquisition module is used for synchronously acquiring neural electrochemical and electrophysiological signals; the main control module is used for carrying out synchronous coordinated control on light stimulation and neural signal detection and completing data communication; the synchronous control module is used for completing the functions of man-machine interaction, data processing, display, analysis and storage, and the like. The system can be used for solving the problems that light control and signal detection are difficult in synchronous and experiment operation is fussy in the research on neurosciences.

The present invention discloses a near-infrared signal-controlled transcranial magnetic stimulation device, comprising a positioning cap, a fNIRS signal acquisition system arranged on the positioning cap, a TMS system and an analysis control device which is connected to the TMS system and the fNIRS signal acquisition system. The near-infrared signal-controlled transcranial magnetic stimulation device utilizes the near infrared signal of the subject to set personalized transcranial magnetic stimulation parameters, avoiding over stimulation or under stimulation. The near-infrared signal-controlled transcranial magnetic stimulation device is simple and easy to operate, is easy to adjust transcranial magnetic stimulation parameters according to the real-time near-infrared signal in transcranial magnetic stimulation to meet the demand of personalized treatment, enhances the stimulation effect of transcranial magnetic stimulation and expands the application scope of near-infrared signal detection, so as to provide a new method and practical tool for neuroscience research, testing and treatment.

Apparatus for applying Transcranial Magnetic Stimulation (TMS) to a patient, the apparatus including a head mount for disposition on the head of a patient; and a plurality of magnet assemblies for releasable mounting on the head mount, wherein each of the magnet assemblies includes a magnet for selectively providing a rapidly changing magnetic field capable of inducing weak electric currents in the brain of a patient so as to modify the natural electrical activity of the brain of the patient; wherein the number of magnet assemblies mounted on the head mount, their individual positioning on the head mount, and their selective provision of a rapidly changing magnetic field is selected so as to allow the spatial, strength and temporal characteristics of the magnetic field to be custom tailored for each patient, whereby to provide patient-specific TMS therapy, to assist in diagnosis or to map out brain function in neuroscience research.

The invention discloses a method for eliminating myoelectricity noise in an electroencephalogram signal based on a single channel. The method is characterized by comprising the steps that firstly, a single-channel electroencephalogram signal is decomposed into a plurality of intrinsic mode components through general average empirical mode decomposition; secondly, blind signal separation s conducted on the intrinsic mode components through multi-set canonical correlation analysis, and a plurality of canonical variables are obtained; finally, the canonical variables with the autocorrelation coefficient lower than a certain threshold value are judged to be myoelectricity noise, the myoelectricity noise variables are removed, and reconstruction is conducted to obtain the electroencephalogram signal with the myoelectricity noise removed. According to the method, the purpose of eliminating the myoelectricity noise in the electroencephalogram signal is effectively achieved from the brand new angle of the single channel, and compared with the traditional blind signal separation technology based on the multiple channels, the myoelectricity noise can be better eliminated. The method is suitable for portable and wearable single-channel and few-channel electroencephalogram devices, is also suitable for multi-channel electroencephalogram devices for clinical diagnosis and neuroscience researches, and significant importance is achieved in further researches of the true physiological activities of the human brain.

The invention discloses a photoelectric regulation and dual-mode detection system for neural information. The system is used for regulating and detecting the neural information of a neural system of an animal and comprises a nerve regulation module, a dual-mode detection module and a main control module; the nerve regulation module is used for applying photic stimulation signals or electric stimulation signals to the neural system and regulating the photic stimulation signals or the electric stimulation signals; the dual-mode detection module is used for detecting electrophysiological and neurochemical dual-mode signals of the neural system; the main control module is used for synchronously and cooperatively controlling stimulation of the nerve regulation module on the neural system and detection of the dual-mode detection module. According to the photoelectric regulation and dual-mode detection system for the neural information, a new research tool and means are provided for neuroscience research, rich information can be obtained, and meanwhile the problems that the experiment efficiency is low and data synchronization treatment is difficult due to the fact that different devices are adopted in photoelectric regulation and signal detection in the neuroscience research are solved.

The invention belongs to the field of cell biology and relates to a method and reagent for the separation and culture of neurons. The method and the reagent are used for solving problems in the prior art, the current in-vitro hippocampal neuron separation and culture methods are improved, and the problem that the proliferation and activity of hippocampal neurons during primary culture can not be maintained is solved. According to the method and the reagent, a relatively mature in-vitro hippocampal neuron culture method is established, the number of obtained nerve cells is sufficient, the growth status is relatively good, and a large number of high-activity hippocampal nerve cells can be separated from mammalian hippocampal tissue, so that the requirements for the primary culture of the hippocampal cells are met, and the demands on experiments of cell biology during neuroscience research can be met.

The invention provides a light-simulated neural electrode device based on a golden wire ball bonding method and a manufacturing method thereof. The method comprises steps: 1, a patterned bottom polyimide insulation layer is manufactured, and square holes are opened; 2, a middle circuit layer is manufactured; 3; a patterned top polyimide insulation layer is manufactured, and square holes and round holes are opened at the same positions; 4; a micro LED bare chip is placed in two layers of polyimide square holes, and instantaneous drying glue is applied to the periphery of the micro LED bare chip; 5, by adopting the golden wire ball bonding method, the micro LED bare chip and a golden circuit layer are connected; 6, epoxy resin glue is applied for realizing packaging; and 7, a sacrificial layer is corroded, and electrode release is completed. The electrode device of the invention mainly aims at providing power for the micro LED bare chip, and light emitting by the LED carries out light simulation on an experimental animal with specific gene transfection; and the process is simple, the LED is arranged flexibly, devices of different forms can be manufactured according to different simulation areas, and a new tool is provided for implantable medical device development and neuroscience research.

The invention provides a super-lens microstructure generation method. The method includes the following steps of generating phase maps, calculating phase distribution and generating microstructures. The invention further relates to a micro biphoton microscopy system based on the super-lens. A super-surface lens is introduced into the field of biphoton microscopy, medium numerical aperture focusingis achieved under full field of view, the structure of a microscope is greatly simplified, the weight of the overall equipment is largely reduced, and lighter load animal experiments can be achieved.The reliability of in vivo biphoton microscopy experimental data is improved, and the method has high scientific value for micro biphoton, especially in vivo microscopic imaging; the influence of a backpack type micro microscope system on observed objects (such as mice) is further reduced. According to the system, from simulation design to processing to experiments, a super-surface lens is introduced into the biphoton microscopy imaging system, and a new generation of imaging equipment is provided for brain imaging of living animals. The progress in brain and Neuroscience research is promoted.

The invention belongs to the field of cell biology and relates to a method and reagent for the separation and culture of neurons. The method and the reagent are used for solving problems in the prior art, the current in-vitro hippocampal neuron separation and culture methods are improved, and the problem that the proliferation and activity of hippocampal neurons during primary culture can not be maintained is solved. According to the method and the reagent, a relatively mature in-vitro hippocampal neuron culture method is established, the number of obtained nerve cells is sufficient, the growth status is relatively good, and a large number of high-activity hippocampal nerve cells can be separated from mammalian hippocampal tissue, so that the requirements for the primary culture of the hippocampal cells are met, and the demands on experiments of cell biology during neuroscience research can be met.

The present invention discloses a brain function experimental task stimulation system with real-time feedback and task update functions. The system comprises a computing platform, for detecting and positioning a brain functional area in real time, monitoring image quality of brain function imaging in real time, and detecting and feed backing the current task response level of the brain; the computing platform is provided with a target brain area positioning module, a data quality monitoring module, a task level detection module and a feedback module; the computing platform is also provided with a data acquisition and real-time transmission module, wherein the original function magnetic resonance image is transmitted to the computing platform after acquisition and reconstruction by using a magnetic resonance scanner; and the computing platform is further provided with a task update module, for presenting new experimental task material by comparing and determining to trigger and control the brain function stimulator. According to the system disclosed by the present invention, efficiency of the statistical determination on the active level of the specific brain area is improved, the total time of brain function signal acquisition is shortened, and the system has great practical significance to improve the scanning efficiency of brain function activity and fast positioning of the functional area in clinical patients, and can be applied to the neurological research related to brain functional area detection.

The invention discloses an implantable multimodal neuromodulation electrode based on a photoelectric technology and a manufacturing method thereof. The implantable multimodal neuromodulation electrode based on the photoelectric technology comprises a base, wherein the base is provided with an electric simulation contact and a light simulation contact; the light simulation contact comprises a light emitting diode; and the light emitting diode comprises an external n electrode, a middle light emitting layer and an internal p electrode. According to the implantable multimodal neuromodulation electrode provided by the invention, electric simulation, light simulation or electric and light combined simulation can be carried out on nerve tissues through adjusting the simulation modes according to different disease cases, so as to reach an optimized treatment effect. The preparation technique provided by the invention is simple, the precision is high, the repeatability is good, and moreover, the unit quantity can be increased through further reducing the size of the simulation unit, thereby improving the resolution, thus, the neuromodulation electrode and the manufacturing method thereof can be widely used for neuroscience research, developing more accurate and more reliable neuromodulation technology in clinical study, researching mechanisms of nervous system diseases such as Parkinson and epilepsy.

Diffusion weighted imaging (DWI) and diffusion tensor imaging (DTI) using a new technique, termed multiplexed sensitivity encoding with inherent phase correction, is proposed and implemented to effectively and reliably provide high-resolution segmented DWI and DTI, where shot-to-shot phase variations are inherently corrected, with high quality and SNR yet without relying on reference and navigator echoes. The performance and consistency of the new technique in enabling high-quality DWI and DTI are confirmed experimentally in healthy adult volunteers on 3 Tesla MRI systems. This newly developed technique should be broadly applicable in neuroscience investigations of brain structure and function.

The invention discloses a comprehensive magnetic resonance imaging device. According to the invention, the cerebral function imaging, the MRI perfusion weighted imaging and the magnetic resonance diffusion imaging are united, so that the assessment for the cerebral injury caused by motor neuron disease and Parkinson disease is assisted, a beneficial judging basis is supplied for illness state judgment, and the accuracy rate of disease judgment is increased; an ALS (amyotrophic lateral sclerosis) patient is accompanied with FTD (frontal temporal dementia), so that the detection means, such as cortex thickness, also is beneficial to the disease diagnose and research; functional magnetic resonance imaging (fMRI) and diffusion tensor imaging techniques have strong complementarity; the combination of the functional magnetic resonance imaging and diffusion tensor imaging techniques has a wide application prospect in neurosciences research; unique brain structure and function information can be supplied.

The invention discloses a novel carbon fiber nanocone electrode as well as a preparation method and application thereof, belonging to the field of electrochemistry and material science. The novel carbon fiber nanocone electrode comprises a carbon fiber, an electrode lead and a glass capillary tube, wherein the carbon fiber is connected with the electrode lead through a conductive adhesive; the front part, as long as 50-100 microns, of the carbon fiber is etched to be of a needle shape, and the tip end of the needle-shaped part has the diameter of 100nm-300nm; one end of the glass capillary tube is pulled to form a tip end with the diameter less than or equal to 1micron; the carbon fiber connected with the electrode lead penetrates into the pulled tip of the glass capillary tube, and is exposed out of the tip end of the glass capillary tube; the electrode lead is exposed out of the other end of the glass capillary tube, and the end is sealed by an AB adhesive. According to the invention, the problem that after a nano electrode is insulated, the tip end cannot be exposed easily is solved radically. The carbon fiber nanocone electrode provided by the invention has the advantages of low noise, high sensitivity and high temporal-spatial resolution, and can be applied to real-time detection on neurotransmitter in synaptic cleft in neuroscience research.

The invention belongs to the field of cell biology and relates to a hippocampal neuron separation and primary culture method and reagent. The existing hippocampal neuron separation and primary culture method is improved aiming at the problems of the prior art, and the problem that the proliferation property and the activity of primary culture of the hippocampal neuron cannot be kept is solved. A relatively mature method for culturing hippocampal neurons in vitro is established, the obtained nerve cells are sufficient, the growth condition is good, a number of hippocampal neurons with high activity can be separated from hippocampus of mammals, the requirement of primary hippocampus cell culture is met, and the requirement of cell biology experiments in neuroscience research is met.

The invention discloses an adeno-associated virus with a variant type capsid protein and application thereof. The adeno-associated virus with the variant type capsid protein AAV9-RB is obtained by inserting an amino acid sequence LADQDYTKTA between Q588 and A589 of capsid protein VP1 of wild type AAV9. The adeno-associated virus with the variant type capsid protein AAV9-RB is the first reported virus body that can not only effectively reverse infection from the axon terminal, but also cross the blood-brain barrier to target brain cells. The invention provides better technical support for neuroscience studies and brain disease treatments, with wide application value and prospect.

This invention relates to an interpolation encephalic medicine-feeding canula for nerve scientific research including an integral structure canula and a fixed base, an integral structural airproof cap and a canula filling core, in which, said base is a staged revolving body and screw thread is lathed on the outer wall of a tube part of the staged revolving body, said sealing cap and the core are like a capsule, a rod extending from the inside of the top of the capsule and screwed on the fixed base is set in the inside wall of the capsule as the filling core of the canula to form an integral structure and the outside diameter of the core is a bit smaller than that of the internal diameter, the length of the core is the same with that of the canula and medical stainless steel or polyurethane are materials of the components.

The invention discloses an electroencephalogram signal simulation generating device which mainly comprises an operation unit, a microcontroller unit, an electroencephalogram data input unit, an indication unit, a power supply unit, a signal conversion unit and a signal conditioning output unit. An output end of the operation unit is connected with the microcontroller unit, the microcontroller unitis connected with an input end of the signal conversion unit, the indication unit is connected with the microcontroller unit, the electroencephalogram data input unit is connected with the microcontroller unit, and an output end of the signal conversion unit is connected with an input end of the signal conditioning output unit. The electroencephalogram signal simulation generating device can be used for outputting continuous weak electroencephalogram signals via multiple independent channels; all the multiple independent channels are low in mutual crosstalk and noise; a plurality kinds of man-made noise can be added to the electroencephalogram signals, and requirements for some neuroscience research occasions can be met; electroencephalogram data files which need to be converted can be changed anytime, and operation parameters can be adjusted in real time according to experiment requirements so as to be adapted to different experimental paradigms.

The invention belongs to the field of cell biology and relates to a method and reagent for the separation and culture of neurons. The method and the reagent are used for solving problems in the prior art, the current in-vitro hippocampal neuron separation and culture methods are improved, and the problem that the proliferation and activity of cortex nerve cells during primary culture can not be maintained is solved. According to the method and the reagent, a relatively mature in-vitro cortex nerve cell culture method is established, the number of obtained nerve cells is sufficient, the growth status is relatively good, and a large number of high-activity cortex nerve cells can be separated from mammalian hippocampal tissue, so that the requirements for the primary culture of the cortex nerve cells are met, and the demands on experiments of cell biology during neuroscience research can be met.

The invention discloses an electrochemical detecting system, which comprises self-reference microelectrode arrays including a plurality of detection sties positioned at the parts to be detected. Reference signals and electrochemical response signals can be acquired simultaneously at the same detection site. An electrochemical surveymeter connected with the self-reference microelectrode arrays is to detect concentrations of chemical substance variation of a plurality of detection sties and output data. The electrochemical detecting system can resolve the problem of consecutively detecting rapid release of cerebral trace neurotransmitters for yonks for researches of neuroscience, and provide a novel research tool and means for researches of neuroscience.

The invention relates to a flexible neural microelectrode based on an oxalis corniculata bionic structure and a preparation method. The structure comprises a flexible electrode substrate, enameled copper wires wrapped by a polyethylene insulating sheath, a PDMS packaging wrapping layer, a silicone sealant at electrode pads and a wire polymerization part, and the like, wherein the connection between the enameled copper wires and the flexible electrode pads is realized through conductive silver paste. Similar to a palm-shaped compound leaf structure composed of three small leaves, the flexible electrode substrate also adopts a similar inverted triangular leaf structure and can be used for synchronous neural signal recording of multi-brain-region cortex. According to the preparation method provided by the invention, the metal recording electrode points and the flexible electrode pads are respectively distributed on different sides of the bottom surface and the top surface through an MEMSpolymer film processing technology, so that vertical connection between the enameled copper wires and the flexible electrode pads is facilitated. The method is simple and easy to implement, the mechanical strength is good, rigid contact with brain tissues can be effectively avoided, and the neuroscience research and application requirements of long-term embedding and collecting of cerebral cortexelectric signals can be met.

Apparatus for applying Transcranial Magnetic Stimulation (TMS) to an individual, wherein the apparatus comprises: a head mount for disposition on the head of an individual; and a plurality of magnet assemblies for releasable mounting on the head mount, wherein each of the magnet assemblies comprises a permanent magnet, and at least one of (i) a movement mechanism for moving the permanent magnet and / or (ii) a magnetic shield shutter mechanism, for selectively providing a rapidly changing magnetic field capable of inducing weak electric currents in the brain of an individual so as to modify the natural electrical activity of the brain of the individual; wherein the number of magnet assemblies mounted on the head mount, their individual positioning on the head mount, and their selective provision of a rapidly changing magnetic field is selected so as to allow the spatial, strength and temporal characteristics of the magnetic field to be custom tailored for each individual, whereby to provide individual-specific TMS therapy, to assist in diagnosis or to map out brain function in neuroscience research.

The invention discloses an ECoG intraoperative brain function positioning method based on clustering and classification algorithms. The method comprises the following steps: firstly, the energy proportion of six layers of wavelet decomposition reconstruction single sub-bands is extracted as a characteristic quantity by employing the functional region boundary clustering algorithm, and the class clusters of all leads are clustered by employing a cohesion-type hierarchical clustering model; secondly, a functional area attribute classification algorithm is adopted, six-dimensional time domain statistics and five-dimensional rhythm energy are used as characteristic quantities, PCA is used for carrying out dimensionality reduction on characteristics, and SVM is used for classifying lead functional area attributes; and finally, the algorithms are integrated to establish an ECoG intraoperative brain function positioning integration algorithm, and the clustered class clusters are matched with the functional area attributes by adopting a self-defined similarity algorithm to complete functional area positioning. The algorithm model has good generalization, can realize accurate and rapid intraoperative brain function positioning clinical application, and can be widely applied to neuroscience researches such as intraoperative brain function positioning based on electroencephalogram analysis.

Apparatus and methods in which very small volumes of biological fluid-borne material, particularly large molecules such as proteins, may be selectively extracted from or delivered to interstitial fluid (in vivo or in vitro) by means of intra-parenchymal micro-probes inserted in the brain. The primary use of the micro-probe is in neuroscience research, clinical diagnostics or treatment of epilepsy and other neurological conditions; it may also be applied to other organs and biological systems. Eventual human clinical applications may include neurosurgical monitoring, functional tracking of devices or materials introduced in a surgical procedure, or cerebro-spinal fluid sampling.

The invention discloses a method for eliminating myoelectricity noise in an electroencephalogram signal based on a single channel. The method is characterized by comprising the steps that firstly, a single-channel electroencephalogram signal is decomposed into a plurality of intrinsic mode components through general average empirical mode decomposition; secondly, blind signal separation s conducted on the intrinsic mode components through multi-set canonical correlation analysis, and a plurality of canonical variables are obtained; finally, the canonical variables with the autocorrelation coefficient lower than a certain threshold value are judged to be myoelectricity noise, the myoelectricity noise variables are removed, and reconstruction is conducted to obtain the electroencephalogram signal with the myoelectricity noise removed. According to the method, the purpose of eliminating the myoelectricity noise in the electroencephalogram signal is effectively achieved from the brand new angle of the single channel, and compared with the traditional blind signal separation technology based on the multiple channels, the myoelectricity noise can be better eliminated. The method is suitable for portable and wearable single-channel and few-channel electroencephalogram devices, is also suitable for multi-channel electroencephalogram devices for clinical diagnosis and neuroscience researches, and significant importance is achieved in further researches of the true physiological activities of the human brain.

The invention belongs to the field of cell biology and relates to a method and reagent for the separation and culture of neurons. The method and the reagent are used for solving problems in the prior art, the current in-vitro hippocampal neuron separation and culture methods are improved, and the problem that the proliferation and activity of hippocampal neurons during primary culture can not be maintained is solved. According to the method and the reagent, a relatively mature in-vitro hippocampal neuron culture method is established, the number of obtained nerve cells is sufficient, the growth status is relatively good, and a large number of high-activity hippocampal nerve cells can be separated from mammalian hippocampal tissue, so that the requirements for the primary culture of the hippocampal cells are met, and the demands on experiments of cell biology during neuroscience research can be met.

The invention discloses an implantable intracranial drug delivery cannula suitable for neurosciences research. The drug delivery cannula comprises a microprocessor, a reflective mirror, a fixing support, an inner cavity, a first drug delivery pipe, a seal sleeve, a second drug delivery pipe and an intracranial body; a drug delivery cannula outlet and a drug delivery hole formed in the drug delivery cannula outlet are formed in one end of the first drug delivery pipe, the inner cavity is formed in the first drug delivery pipe, light guiding fibers are arranged between the inner cavity and the inner side of the first drug delivery pipe, and the reflective mirror is arranged at one ends of the light guiding fibers; the microprocessor is arranged at the other end of the first drug delivery pipe, one side of the first drug delivery pipe is provided with the second drug delivery pipe and a drug delivery cannula inlet formed in one end of the second drug delivery pipe, and the drug delivery cannula inlet is sleeved with the seal sleeve. According to the implantable intracranial drug delivery cannula suitable for neurosciences research, a cannula material mets the requirement of medical implantation, fixity and leakproofness are good, and a cannula inner core is high in operability and intellectuality.