An ink supply tube of the present invention comprising an inner layer to come into contact with ink, a middle layer having at least three layers, and an outer layer, wherein the middle layer includes a first middle layer, a second middle layer, and a third middle layer, the inner layer comprises one of an ethylene-tetrafluoroethylene copolymer resin, a tetrafluoroethylene-perfluoroalkoxy alkane copolymer resin, and a modified perfluoroalkoxy-based resin, each having a solvent resistance, an oxygen barrier property, and a low moisture permeability, the first middle layer comprises a polyamide resin having a fusibility with the inner layer or the second middle layer, the second middle layer comprises an ethylene-vinyl alcohol copolymer resin having an oxygen barrier property, the third middle layer comprises an adhesive polyolefin resin having a fusibility with the second middle layer or the outer layer, and having a low moisture permeability, and the outer layer comprises a thermoplastic resin or elastomer. Accordingly, an ink supply tube for an inkjet printer is provided that has a more excellent oxygen barrier property and a more excellent low moisture permeability in addition to an excellent solvent resistance and an excellent flexibility.