The invention relates to the field of cultivation of edible mushrooms, in particular to edible mushroom inoculation equipment. The edible mushroom inoculation equipment comprises a rack, a conveying belt arranged on the rack, a driving motor driving the conveying belt to circularly move through transmission parts, as well as tension devices used for adjusting the degree of tightness of the conveying belt, wherein a punching device, a sizing device, a film sealing device, a film cutting device and a film pressing device are sequentially arranged on the rack in the conveying direction of the conveying belt; a mushroom stick loading station is arranged on the upstream of the punching device, an inoculation station is arranged between the punching device and the sizing device, and an unloading station is arranged on the downstream of the film pressing device. According to the edible mushroom inoculation equipment, an assembly line is adopted, edible mushrooms can be cultivated in an assembly line manner, procedures including punching of mushroom sticks, sizing, film sealing and the like can be completed automatically except loading, inoculation and unloading procedures required to be performed manually, accordingly, the inoculation efficiency of the edible mushrooms is greatly improved, the yield is increased, the productivity is liberated, and the labor intensity of workers is reduced.