The invention discloses a deep water sample sampling device used for a water conservancy reservoir. The deep water sample sampling device mainly comprises a fixed device, a sampler, a sampling device,a control device and a power supply device, wherein the fixed device is located at an internal vertical center position of the sampling device, and the fixed device comprises a fixed frame, a slidingboard, a track bar, a moving board, a water guide pipe, a water transmission channel and a spring bolt; the sampler is located at the inside of the fixed device, and the sampler comprises a water inlet, an obstructing device, a groove and an electric isolating board; left and right sides of the bottom of the sampling device are provided with limiting parts, and a water feeding device is arrangedunder the sampling device; the water feeding device comprises a hydraulic adjusting board, a protective mesh, a pushing board, a water feeding channel and a baffle; left and right sides of an externalwall of the sampling device are provided with balance weight parts, and an upper end of the sampling device is provided with a buoyancy force part. In a word, the deep water sample sampling device used for the water conservancy reservoir is simple in structure and convenient to operate, guarantees stability of an acquisition process and can sample deep water at different directions.