33 results about "Phosphoglycerate kinase 1" patented technology

The invention discloses a high-resistance yeast strain resistant to high temperature, high sugar and freezing and a construction method thereof. The high-resistance yeast strain selects a strong promoter PGK1 to overexpress the H / ACA small nucleus in the parent yeast strain Kernel RNA (SNR84) full sequence to obtain the high tolerance yeast strain. Compared with the parental strain, the cell activity of the strain is significantly improved in high temperature, high sugar and freezing environments. The baker's yeast has strong resistance to high temperature in heat shock treatment, strong resistance to high sugar in high-sugar dough, and strong resistance to freezing in frozen dough, which solves the technical obstacles and quality defects in the bread making process, and has a wide range of Application prospect.

The invention discloses a blood marker-PGK1 for rheumatoid arthritis clinical diagnosis, and discloses a reagent for rheumatoid arthritis clinical diagnosis. The reagent comprises a PGK1 antibody, an HRP-IgG antibody and a conventional reagent in a blood diagnosis reagent. The conventional reagent in the blood diagnosis reagent comprises a carbonate buffer solution, PBST washing liquor, confining liquid, a developing solution and a stop solution. The prepared detection reagent has the advantages of being high in sensitivity, high in specificity and the like and is capable of being widely applied to preliminary survey and health examination of clinical rheumatoid arthritis and providing a reliable basis for clinical diagnosis.

The invention discloses a baker's yeast strain suitable for dough fermentation without adding sugar and a breeding method thereof, belonging to the technical field of bioengineering. The present invention obtains the fast-fermenting baker's yeast strain by completely knocking out the glucose phosphomutase gene PGM2 in the parental baker's yeast strain, and selecting the strong promoter PGK1 to overexpress the whole sequence of H / ACA small nucleolar RNA (SNR84) . The fast-fermenting baker's yeast strain was subjected to 1h CO in unsweetened dough fermentation 2 Gas production reached 818mL, which was 21.2% higher than that of the parental strain (the parental strain was fermented without sugar for 1h CO 2 Gas production is 675mL), and the fermentation time is shortened by 18.0%. The baker's yeast has strong fermentation ability in dough without adding sugar and has wide application prospects.

The invention relates to a protein surface display system and specifically provides a surface display system taking saccharomyces boulardii as an expression heterologous protein of the surface display system. The saccharomyces boulardii of the system is purchased from Laboratoires Biocodex in France, the registration certificate number of an imported drug is s20040038, an AGA1 gene and an AGA2 gene are obtained by amplification in a DNA (deoxyribonucleic acid) group, a pPIC9AGA2 plasmid is taken as a template for amplification of an AGA2 gene expression core, and the AGA2 gene of the saccharomyces boulardii is used for replacing the AGA2 gene in the AGA2 gene expression core. The obtained AGA1 gene and the AGA2 gene expression core after replacement are connected onto a basic plasmid pSP and respectively controlled by over-expression promoters TEF1 and PGK1, and then a general surface display expression plasmid pSDSb which can express on the surface of the saccharomyces boulardii can be further obtained. The system disclosed by the invention can continuously express the heterologous protein which is fused with Aga2p, be displayed on the surface of the saccharomyces boulardii, further realize the over-expression of the certain heterologous protein, take the heterologous protein as a pathogen live vaccine carrier, further research the interaction between the proteins and realize the actions which can not be replaced by other microorganism carriers.

A freezing-resistant yeast strain for bread fermentation and a breeding method thereof. The yeast strain provided by the invention is saccharomyces cerevisiae BT-N with an accession number of CGMCC No.7151. When other fermentation performance is not influenced, the strain has a cell freezing survival rate of 92.75% after being frozen at -20 DEG C for 21 days and has a relative fermentation capacity of 53.24%, which are increased by 57.39% and 24.78% respectively when compared with parent strains (who have a freezing survival rate is 35.36% and have a relative fermentation capacity of 28.46%). The strain is realized by knocking out of the neutral trehalase coding gene NTH1 of saccharomyces cerevisiae CICC31616, and overexpression of the trehalose-6-phosphate synthase coding gene TPS1 by selecting a strong promoter PGK1. The bred yeast strain has no special requirements for fermentation equipment or conditions, can be used by equipment and under conditions of common factories, and has wide application prospects.