The invention discloses an attapulgite sterilization mite suppressor and a preparation method thereof. The attapulgite sterilization mite suppressor comprises the following active ingredients in parts by weight: 50-100 parts of modified attapulgite, 4-8 parts of radix stermonae, 5-10 parts of sophora flavescens, 3-6 parts of fructus cnidii, 5-10 parts of zanthoxylum root, 2-5 parts of cortex phellodendri, 3-6 parts of fructus kochiae, 5-10 parts of leaf of hongkong kumquat, 4-8 parts of cercis bark, and 6-12 parts of paulownia flower. All the active ingredients are combined, the attapulgite sterilization mite suppressor has mite killing and suppression and bacteria inhibition functions, not only is good in mite killing effect and eradicates the breeding of mites, but also can effectively suppress the breeding of microorganism in the environment, and can achieve the bacteria inhibition rate by more than 95%, is safe and mild, free of toxic and side effect, as well as from adverse effect, and can be widely applied to products of soaps, shower gel, liquid shampoo, disinfectant, laundry detergent, facial cleanser and the like.