2results about How to "High dehydrogenation activity" patented technology

This invention relates to the field of organic synthesis technology, and discloses a copper-based catalyst, its preparation method, and a method for catalyzing the preparation of cyclododecanol from cyclododecanol into cyclododecanone. The preparation method of the copper-based catalyst includes the following steps: preparing a catalyst with a specific surface area of ​​200-250 m² / g. 2 / g Al2O3-SiO2 composite support; copper source and rare earth oxide additives were loaded onto the Al2O3-SiO2 composite support, dried and calcined to obtain catalyst precursor; the catalyst precursor was reduced to prepare Cu + A copper-based catalyst with a molar ratio of 1.8 to 2.5:1 to elemental copper. This invention can control the Cu content in the copper-based catalyst. + The molar ratio with elemental copper allows the catalyst's activity state to be adjusted according to different production needs, thereby enabling flexible control of the reaction rate, improving process adaptability and operational stability; at the same time, it helps to suppress side reactions and improve the selectivity and purity of cyclododecanone.

The application belongs to the technical field of biocatalytic synthesis of steroid drugs, and particularly relates to a dehydrogenase, a gene, an engineering bacterium and application, the amino acid sequence of the dehydrogenase is SEQ ID NO. 1 or SEQ ID NO. 2, the dehydrogenase has high dehydrogenation activity, high stereoselectivity and strong regioselectivity, and no other conformation impurities are generated; the engineering bacterium can efficiently secrete the dehydrogenase, and effectively improves the conversion efficiency of a substrate, especially for a substrate 6-methy format (11beta, 17alpha-dihydroxy-6alpha-methylpregna-1, 4-diene-3, 20-diketone), which is low in cost and has significant industrialization potential; the dehydrogenase mutant has clear physicochemical properties, and the method of whole cell catalysis is used, so that the cumbersome step of enzyme purification is omitted, and the cost is reduced and the operation is simple.