The invention discloses a high energy hybrid thulium-doped pulse laser single-frequency amplifier which comprises a seed resource, a wavelength division multiplexer, an optical fiber pumping source, a polarization-maintaining single-mode single-cladding thulium-doped optical fiber, an isolator, a beam splitter, an output end, a filter, an acoustic optical modulator, a semiconductor pumping source and a laser crystal. The high energy hybrid thulium-doped pulse laser single-frequency amplifier has the advantages of being simple in structure and stable in system, the optical fiber-solid combined method is used, optical fiber amplification is adopted for a prime stage, the capacity for amplifying small signals is effectively improved, crystal amplification is adopted for a post stage, the advantage that solid lasers are prone to generating low-multi-pulse pulsed light is developed, the nonlinear effect easily occurring in optical fiber amplifying under high power is effectively avoided, low-multi-pulse high-energy pulse output is accordingly achieved, and the good application aspect is achieved.