The invention belongs to the technical field of feed, and particularly relates to a feed additive capable of improving the high-temperature stress resistance ability of a mariculture animal, and a preparation method and application thereof. The feed additive comprises chromium nicotinate, various vitamins, various trace elements, glutamine, cysteamine hydrochloride, allicin, astragalus polysaccharide, berberine hydrochloride, bacillus subtilis, lactic acid bacteria, sodium bicarbonate, methionine, lysine, tryptophan, potassium dihydrogen phosphate and digestive enzyme, and is added into mariculture animal feed at an addition ratio of 0.1-0.5%, the high-temperature stress resistance ability of the mariculture animal can be effectively improved, the ingestion rate, the digestion rate, the growth rate and the survival rate of the mariculture animal are obviously improved, and the physiological steady state and the immunity of the mariculture animal are improved. Meanwhile, raw materials adopted by the feed additive have a stable source and a reasonable price cost, and the feed additive disclosed by the invention has a simple production technology and does not have any bad influence onfeed palatability.