The invention discloses a manufacturing method of an interlayer film. The method comprises the following steps of step1, providing a semiconductor substrate forming a pattern structure; step2, using afirst insulating layer which has a high filling ability and is formed through a first growth technology to fill a pattern interval region and extending the first insulating layer to an outer portionof the pattern interval region, and forming a closed cavity while filling; step3, taking a pattern structure as an end point of grinding to carry out first chemical mechanical grinding, forming a dishing defect in a pattern structure region and at the same time, opening a cavity; step4, forming a second insulating layer by using a second growth technology, using the second insulating layer to completely fill the cavity, wherein hardness of the second insulating layer is higher than the hardness of the first insulating layer; and step5, taking the pattern structure as the end point of grindingto carry out second chemical mechanical grinding, and eliminating the dishing defect by using a characteristic that the hardness of the second insulating layer is increased. In the invention, the cavity formed through filling an interlayer film in the pattern interval region can be eliminated, and the dishing defect on an interlayer film surface of a top of the pattern interval region can be reduced or eliminated.