The invention relates to a radiopharmaceutical passive
skin anaphylaxis test method, which comprises the following steps: animal grouping: dividing test animals into a
positive control group, a
negative control group and a
drug group, and marking; preparing the
medicine: respectively mixing the negative reference substance, the positive reference substance and the radiopharmaceutical with the
adjuvant to obtain each group of mixed
emulsion; preparing
antibody serum: taking a plurality of test animals from each group, performing
drug injection, sampling blood on the fourteenth day after the last
sensitization, separating the serum, and diluting to obtain
antibody serum with medium, high and low concentrations; passive
sensitization: taking a plurality of normal test animals from each group, injecting corresponding
antibody serum into each group, and carrying out passive
sensitization; and excitation: after sensitization is carried out for 48 hours, injecting each group of solutions containing dye liquor into each group of test animals for excitation, measuring the size of blue spots, and determining that the test animals with the
diameter greater than 5 mm are positive. The method can be used for safely, effectively, simply and conveniently carrying out preclinical evaluation research on the sensitization of various radiopharmaceuticals.