The invention provides a chemical wastewater reaction tank wastewater sampling device, and relates to the field of chemical wastewater treatment. The chemical wastewater reaction tank wastewater sampling device comprises a sampling barrel, a sleeve is fixedly mounted in the sampling barrel, a telescopic sealing gasket is fixedly mounted at the bottom end of the sampling barrel, a rotating mechanism is rotatably connected into the sleeve, and a pressing mechanism is rotatably connected into the rotating mechanism, penetrates through the sampling barrel and extends to the position above the sampling barrel; and the sampling barrel is divided into a stirring cavity, a water storage cavity and an exhaust cavity, and the stirring cavity is located at the bottommost part. According to the chemical wastewater reaction tank wastewater sampling device, by arranging the center rod and the spiral steel bar, wastewater in the stirring cavity can be stirred through vertical movement of the center rod, air in the water storage cavity is pumped out through the piston sleeve to form negative pressure, and after the threaded shaft is rotated, the stirred wastewater can be automatically stored in the water storage cavity, so that the problems that in the prior art, a reaction tank is large in area, stirring is not easy, and stratified sampling operation is inconvenient are solved.