The invention discloses a preparation method of a graft modified fish
protein-
sugar coupled compound, which comprises the following steps: dissolving
protein in a high-ionic-strength
buffer solution, and performing high-speed shearing to obtain a uniform myofibrillar
protein solution; subjecting the myofibrillar
protein solution to high-pressure homogenization pretreatment to expand protein, so that the alpha-
helix content of the myofibrillar
protein solution is increased,
glycosylation sites of the myofibrillar
protein solution are increased, then subjecting the myofibrillar protein solution and
glucosamine to a wet
glycosylation reaction in a water phase, and stabilizing the natural
protein structure and improving the
glycosylation degree by utilizing a macromolecular
crowding theory; and performing
dialysis desugaring on a product obtained after the glycosylation reaction, and performing vacuum
freeze drying to obtain the grafted modified protein-
sugar coupled compound disclosed by the invention. Compared with a traditional glycosylation mode, the preparation method disclosed by the invention has the advantages that the
grafting degree of the myofibrillar protein and the
glycosyl donor and the
solubility of the myofibrillar protein in a low-
ion solvent can be remarkably improved, and the
oxidation resistance of the myofibrillar protein is also improved.