The invention discloses a colistin sulfate production method, which sequentially comprises the following steps: filtering, calcium and magnesium removing-absorption desorption, concentration, desalting, neutralization and spray drying, and is characterized in that the filtering adopts ceramic membrane ultra-filtration filtering, the absorption desorption adopts a continuous ion exchange system filled with a methacrylic acid type carboxylic acid base resin or an acrylic acid carboxylic acid base resin to carry out absorption desorption, and the concentration adopts nano-filtration concentration. The production method has the following advantages that: colistin sulfate is an intracellular product, and cells can be broken when filtration is performed by using a ceramic membrane, such that the colistin sulfate in the colistin sulfate cells can be collected; the equipment is compact, the system is simplified, the pipeline is reduced, and the occupation area is small; the resin consumption is reduced; and the adopted nano-filtration has advantages of high concentration ratio, low energy consumption and the like, and the whole method has advantages of high yield, good purification effect, low production cost and the like.