The invention discloses a Testis et Penis Callorhini refining method. According to the method, the content of extracted low-molecular polypeptide, amino acid, trace elements and the like is higher, the defects of labor and time consumption, crude medicine waste, inconsistent quality, low extraction rate, damage to nutrients and the like of the traditional processing method, decoration method, reflux extraction method and the like are overcome, a special deodorization and bitterness removal method is adopted, and a Testis et Penis Callorhini extract with better color and taste is obtained; Testis et Penis Callorhini has effects of replenishing essence, benefiting marrow, tonifying the kidney and strengthening yang when reasonably cooperating with wolfberries, epimedium, prepared rehmannia roots, Szechwan lovage rhizomes, two-toothed achyranthes roots, raspberry fruits, dodder seeds, oriental waterplantain rhizomes, tree peony bark, amur cork tree bark, cinnamon, white peony roots, Chinese yams, eucommia bark, Angelica sinensis, wolfiporia extensa, morinda officinalis, desertliving cistanche, dried orange peels and galangal for use, can be used for treating kidney yang deficiency, impotence, prospermia, kidney essence deficiency, soreness and weakness of waists and knees and the like and has the ideal heath care effect.