The invention discloses a detection head of a spectrophotometer, relating to the spectrophotometer. The detection head of the spectrophotometer has small volume and high sensitivity, can be separated with an instruement host and is played once plugged, is portable; and with the adoption of the detection head of the spectrophotometer, the interference of stray lights is reduced, the immediate detection requirements in field and site are satisfied, and the detection stability can be greatly enhanced. The detection head of the spectrophotometer comprises a shell, an incidence optical fiber, a reference optical fiber, an incidence optical fiber collimator, a light splitting part, an optical reflection part, a return optical fiber collimator, a return optical fiber and a sample chamber. Because an optical fiber type probe is adopted, the anti-interference performance is enhanced with optical fiber transmission. Because a conical reflecting prism is adopted, an integrated multi-optical-pathabsorption optical detection head which has compact structure can be obtained, and therefore, under the condition without increasing the length of a sample chamber, an absorption optical path is enlarged, the interference of stray lights is reduced, the sensitivity is enhanced, and the optical regulation difficulty is also lowered; while, spectrophotometers at home and aboard are all in a single absorption optical path optical detection head. Because a reference optical path is arranged, the detection stability can be greatly enhanced.