The invention discloses a method for measuring solvent volume by optical fiber. First, a main controller is connected to an optical fiber probe, a reagent bottle is placed in a water tank, the optical fiber probe extends into the water tank, and is placed symmetrically on both sides of the reagent bottle; Solvent, the optical fiber probe collects the data of N times of photometric feedback values, and then obtains the first collected data Q=the sum of N times data / N, and saves it as the initial value; when the volume is fixed, the optical fiber probe collects the current data Q 1 , the main controller will get the X from the initial value / current data 1 Compare with trigger value, if X 1 If it is less than the trigger value, then collect N times of data again, and set the ratio of initial value / current data to X 2 Compare with trigger value, if X 2 If the value is greater than the trigger value, the false trigger delay is prevented. After the delay, the fiber probe collects N times of data, and the ratio of the initial value / current data is X 3 Compare with trigger value, if X 3 If it is greater than the trigger value, it is judged that the solvent has reached the required measurement volume. In this case, the step response is used for constant volume, and the measurement speed is fast and the accuracy rate is high.