The embodiment of the invention provides an aqueous environmentally-friendly nano-copolymer multifunctional defoamer and a preparation method thereof. The raw materials of the defoamer comprise, by mass, 20-26% of a natural vegetable fatty alcohol, 10-15% of a higher fatty alcohol, 5-10% of an amide, 5-8% of a nano-hydrophobic agent, 0.5-1% of a fluorine monomer, 3-6% of a silane, 2-5% of an initiator, 1-2% of an active polymerization agent, 0.5-1% of a catalyst, 1-2% of a thickener, 1-2% of an antifreeze agent and 30-45% of deionized water. The defoamer provided by the embodiment of the invention is prepared by raw material selection and a copolymerization synthesis mode, a nano-material group is introduced to change the polymer structure, and harmful substances such as a conventional emulsifier, formaldehyde and silicone oil are not used, so the versatility, the weather resistance, the stain resistance, the high corrosion resistance and the auto-cleaning property are improved. The product can be decomposed by hydrolysis, photodegradation and biodegradation without causing cumulative effects, so the product is very safe to water sources and the environment.