The invention discloses a processing method for Trichosanthes kirilowii seed oil. The processing method comprises the following steps that firstly, crushing and enzymolysis are conducted, wherein thewater content of Trichosanthes kirilowii seeds is controlled to be, by weight, 8-12%, and the Trichosanthes kirilowii seeds are crushed and mixed with a mixing solution A for an enzymolysis reaction;secondly, screw pressing is conducted, wherein the material obtained in the first step is pressed, and Trichosanthes kirilowii seed cakes and pressed oil are obtained; thirdly, filtration and separation are conducted, wherein the pressed oil is filtered, cooled and filtered, and the Trichosanthes kirilowii seed oil is obtained. The processing method for the Trichosanthes kirilowii seed oil has theadvantages that the extraction rate of the Trichosanthes kirilowii seed oil is high, energy conservation and environment protection are achieved, no pollution is produced, the pressing time is short,the instantaneous pressing pressure is low, the Trichosanthes kirilowii seed oil does not need to be subjected to chemical refining, and the production cost is low, and the large-scale, industrial and continuous production of the Trichosanthes kirilowii seed oil is achieved.