The invention belongs to the field of weaving of textile products, in particular to an antibacterial and anti-radiation pure silk fabric and a preparation method thereof. The antibacterial and anti-radiation pure silk fabric consists of warp yarns and weft yarns, wherein each warp yarn is prepared from the following components in parts by weight: 40 to 50 parts of mulberry silk, 5 to 8 parts of modified resin cotton, 2 to 3 parts of nanometer tencel, 1 to 4 parts of wool fabric, 3 to 5 parts of bamboo pulp fiber, and 4 to 5 parts of milk protein fiber; each weft yarn is prepared from the following components in parts by weight: 40 to 50 parts of the mulberry silk, 2 to 5 parts of spandex, 2 to 3 parts of modified resin cotton, 4 to 8 parts of stainless steel fiber, 5 to 6 parts of chitosanfiber, 3 to 5 parts of nanometer tencel, 2 to 4 parts of PTT (polytrimethylene terephthalate) fiber, and 1 to 4 parts of soy protein fiber. The preparation method comprises the following steps of respectively weaving the warp yarns and the weft yarns; weaving the weft yarns and the warp yarns on a jet loom; arranging; finally, drying, washing by water, and airing, so as to obtain the antibacterial and anti-radiation pure silk fabric.