The invention provides a phosphorylated
krill peptide. A preparation method comprises the following steps: (1)
degreasing krill powder, then adding a NaOH solution into the
krill powder and centrifuging to obtain a supernatant, regulating a pH value of the supernatant until reaching the isoelectric points of
protein and centrifuging to obtain a precipitate; (2) preparing the krill
protein precipitate into an
aqueous solution, adding a
phosphate, regulating the pH value of the solution and performing
spray drying on the solution so as to obtain krill
protein powder; standing the krill protein powder at a high temperature so as to obtain phosphorylated krill protein; (3) repeatedly washing the phosphorylated krill protein with
distilled water and centrifuging; and (4) preparing the phosphorylated krill protein into the
aqueous solution, adding 2M NaOH, regulating the pH value of the solution, adding a
protease into the solution, hydrolyzing for 3 to 6 hours at a proper temperature, heating to 100 DEG C and stopping an
enzyme reaction, centrifuging to obtain the supernatant and performing the
spray drying on the supernatant so as to obtain the phosphorylated krill
peptide. The animal experiment proves that the prepared phosphorylated krill
peptide can be used for obviously regulating the bone
metabolism level of a model rat with the
osteoporosis so as to enhance the
bone mineral density and improve the bone biomechanical property, thereby having obvious functions for preventing and treating the
osteoporosis.