The invention discloses a high-temperature resistant automobile air-conditioning wiring harness and a preparation method thereof. The high-temperature resistant automobile air-conditioning wiring harness consists of two parts, namely a wiring harness shell and a thermal-insulating membrane, wherein the wiring harness shell consists of natural rubber, a PEEK (Polyetheretherketone) material and polyvinyl chloride; the thermal-insulating membrane consists of silicon carbide powder, a high-temperature resistant fiber, boron phosphide, polyethylene and a high-temperature adhesive. As the wiring harness shell is made of three raw materials, namely the natural rubber, the PEEK material and the polyvinyl chloride, as master batch, the strength of the wiring harness shell can be improved, and the preparation efficiency can be improved; the constitution components, namely the silicon carbide powder, he high-temperature resistant fiber, the boron phosphide, the polyethylene and the like, of the thermal-insulating membrane, are very good in thermal resistance, so that the thermal resistance of the prepared thermal-insulating membrane is improved; finally by using the high-temperature adhesive,the wiring harness shell and the thermal-insulating membrane can be well adhered, and due to a water cooling mode, the toughness of the thermal-insulating membrane can be ensured.