58 results about "Morpholino Oligos" patented technology

The present invention provides a kit and a method for targeting of a diagnostic or therapeutic agent to a target site in a mammal having a pathological condition. The kit comprises, in separate containers, (a) a first conjugate comprising a targeting moiety and a Morpholino oligomer, wherein the targeting moiety selectively binds to a primary, target-specific binding site of the target site or to a substance produced by or associated with the target site; (b) optionally, a clearing agent; and (c) a second conjugate comprising a complementary Morpholino oligomer and a diagnostic agent or therapeutic agent. The method comprises administering (a), optionally (b), and (c) to a mammal.

This invention provides quinazoline derivatives represented by the structural formula: (I); wherein: R₂ is hydrogen, NR′R″, C_1-7 alkyl, arylC_1-7 alkyl or C_3-10 cycloalkyl; R₄ is amino, C_1-7 alkyl, C_2-7 alkenyl, C_3-10 cycloalkyl, C_3-10 cycloalkenyl, aryl, heterocyclic, arylalkyl, heterocyclic-substituted C_1-7 alkyl or C_3-10 cycloalkyl-C_1-7 alkyl; R₅ is hydrogen or C_1-7 alkyl, or R₅ and R₄ together with the nitrogen atom to which they are attached form a heterocyclic ring; Y is a single bond, C_1-7alkylene, C_2-7 alkenylene or C_2-7 alkynylene; R₆ is halogen, heteroaryl or aryl; R′ and R″ are each independently hydrogen, C_1-7 alkyl-carbonyl or C_1-7 alkyl; provided that R₄ is not phenyl substituted with morpholino when R₂ is H and R₅ is H, and provided that when NR₄R₅ is piperazinyl, said NR₄R₅ is either non-substituted or substituted with methyl or acetyl; a pharmaceutically acceptable addition salt, a stereoisomer, a mono- or a di-N-oxide, a solvate or a pro-drug thereof, for the treatment of viral infections.

The invention provides antisense antiviral compounds and methods of their use and production in inhibition of growth of viruses of the Flaviviridae, Picornoviridae, Caliciviridae, Togaviridae, Arteriviridae, Coronaviridae, Astroviridae and Hepeviridae families in the treatment of a viral infection. The antisense antiviral compounds are substantially uncharged morpholino oligonucleotides having a sequence of 12-40 subunits, including at least 12 subunits having a targeting sequence that is complementary to a region associated with stem-loop secondary structure within the 5′-terminal end 40 bases of the positive-sense RNA strand of the virus.

Morpholino-based oligomers suitable as antisense agent comprising modifications of phosphorodiamidate backbone or modification with 5-substituted pyrimidines of morpholino compound that is soluble in culture medium and sufficient for cell penetration thereby eliminating the need for injecting into the cells. Monomers comprising the said oligomers and its method of manufacture, method of manufacture of the said oligomers and its dye, flurophore, drug, biomolecule conjugate wherein the said oligomers find different end use but not limited to regulation of gene expression, tissue culture with improved transfection efficiency and related studies on cellular transfection.

The invention provides sense antiviral compounds and methods of their use in inhibition of growth of viruses of the Flaviviridae, Picornoviridae, Caliciviridae, Togaviridae, Coronaviridae families and hepatitis E virus in the treatment of a viral infection. The sense antiviral compounds are substantially uncharged morpholino oligonucleotides having a sequence of (12-40) subunits, including at least (12) subunits having a targeting sequence that is complementary to a region associated with stem-loop secondary structure within the 3′-terminal end (40) bases of the negative-sense RNA strand of the virus.

The invention relates to a fluorescent probe used for conducting linkage detection on superoxide anions (O2.-) and hydrogen polysulfide, in particular to a fluorophore derivative for detecting superoxide anions and hydrogen polysulfide and application thereof. The fluorophore derivative is shown in the general formula I. In the general formula I, R1 represents a C1-22 alkyl group or a halogenated benzyl group or C1-C12 organic acid ester chains; R2 represents hydrogen, a sulfonic group or an amino group or a carboxyl group or a nitro group or halogen; X (a positioning functional group) represents -OH, a butyl triphenyl phosphonium group, an N-propyl morpholino group, a biotin group, a folic acid group, a glycosyl chain group, benzyl chloride or a C1-22 alkyl group; Y1 represents N, O or S; Y2 represents N, O or S. In the presence of the superoxide anions (O2.-) and the hydrogen polysulfide, corresponding fluorescence emission wavelength and intensity change, and the fluorescent probe can be used for detecting the superoxide anions (O2.-) and the hydrogen polysulfide in organisms, interference of external detection conditions can be greatly reduced, the detection signal to noise ratio is high, and sensitivity and selectivity are good. The fluorophore derivative has important significance in biomedicine.

The invention provides a perfusion stock composition, for preserving a donor organ for transplantation, comprising: a source of 60 to 100 mM Na⁺; a source of 10 to 20 mM K⁺; a source of 5 to 10 mM Mg²⁻; a source of 0.25 to 0.75 mM Ca²⁺; 10 to 40 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris or THAM), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 3-(N-morpholino)propanesulfonic acid (MOPS), 2-(N-morpholino)ethanesulfonic acid (MES), N,/N-bis-(2-hydroxyethyl)-2-aminoethansulfonic acid (BES), or N/-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES); a source of 10 to 30 mM HCO₃⁻; 1 to 30 mM glucose; 1 to 20 U/L insulin; 1 to 10 mM fructose diphosphate or a salt thereof; 1 to 40 mM aspartate or glutamate; 1 to 10 mM adenosine, cAMP or cGMP; 1 to 10 mM reduced glutathione; and 30 to 100 mM lactobionate or mannitol; and optionally a diluent. The invention also provides a perfusion composition, a kit, a method, and a perfusion apparatus, each related to the perfusion stock composition.

This invention provides pH buffered plant nutrient compositions, methods for fertilizing a plant growing or a seed germinating in a hydroponics system, methods for growing a plant in a hydroponics system, and methods for making a pH buffered plant nutrient composition. The compositions and methods of this invention are useful with distilled water, deionized water, filtered water, and United States municipal tap water. The compositions and methods of this invention are useful with most of the municipal water supplies in the United States. pH buffering agents useful in the practice of this invention include phosphate buffers, aquarium buffers, 2-[N-morpholino]ethanesulfonic acid, and mixtures thereof.

The invention relates to a promega for testing glycolated hemoglobin by using an enzymatic method, which contains the ingredients of hemolysis buffer: 10-1000 mmol/L of N-chclohexyl-2 ethyl amine sulfoacid, 10-1000mmol/L of morpholino propane sulfoacid, and 1-100g/L of polyethylene glycol oxide dodecyl ether; reagent R1a: 10-1000kU/L of pepsin of pig, 0.1-10mmol/L of 2-(iodophenyl)-3-(2, 4-dinitro benzene)-5-(2, 4-sulfophenyl)-2H tetrazole sodium salt, and 0.0001-0.1mol/L of HCl; reagent R1b: 0.1-10mmol/L of morpholino ethane sulfonic acid, and 0.5-50mmol/L of CaCl2; neutralization buffer: 0.0001-0.1mol/L of NaOH reagent, 2:1-1000kU/L of fructose valine oxidase, 30-600kU/L of peroxydase, 0.01-0.5mmol/L of N-(carboxymethyl aminocarbonyl)-4,4 quadri (dimethyl amine)-diphenylamine sodium salt, and 10-1000mmol/L of Tris-HCl. The promega has the zymohydrolysis effect which is better than alkalescence prolease, little dosage, low cost and accurate measuring result.

The invention discloses a synthetic medium for Staphylococcus carnosus, and a preparation method and application of Staphylococcus carnosus fermentation broth. The synthetic medium for Staphylococcus carnosus comprises the following raw materials by weight: on the basis of a volume of 1 L, 10 to 50 g of anhydrous glucose, 0.1 to 1.5 g of lysine, 1.0 to 5.0 g of valine, 0.1 to 1.5 g of glycine, 1.0 to 5.0 g of arginine, 1.0 to 5.0 g of proline, 5 to 10.0 g of glutamic acid, 0.1 to 0.5 g of tryptophan, 0.1 to 1.5 g if cystine, 1.0 to 3.0 mg of thiamine hydrochloride, 1.0 to 3.0 mg of calcium pantothenate, 1.0 to 3.0 mg of nicotinic acid, 1.0 to 3.0 mg of manganese sulfate, 0.1 to 1.0 g of potassium dihydrogen phosphate, 0.1 to 0.8 g of magnesium sulfate heptahydrate, 0.005 to 0.010 g of ferrous sulfate heptahydrate, 1.0 to 5.0 g of dipotassium hydrogen phosphate trihydrate and 6 to 10 g of 3-(N-morpholino)propanesulfonic acid, with the balance being water. The invention also discloses a preparation method for the Staphylococcus aureus fermentation broth. The synthetic medium for Staphylococcus carnosus is reasonable in composition, rich in nutrients and capable of meeting the growth demands of Staphylococcus carnosus. The growth amount of Staphylococcus carnosus obtained through the synthetic medium provided by the invention is increased by 5% compared with the growth amount of Staphylococcus carnosus obtained through frequently used LB (Luria-Bertani) mediums.

A compound of Formula (I):

or a salt or solvate thereof, wherein:

• • One of R¹ and R² is H and the other represents —NHCONHR⁴,
  • wherein R⁴ represents
    • a phenyl or naphthyl group which may be optionally substituted by one or more substituents independently selected from —C_1-6 alkyl, —C_1-6 haloalkyl, halogen, C_1-6 alkoxy, C_1-6 haloalkoxy, OH, NO₂,
    • C_3-7 cycloalkyl, indanyl, or
    • R⁴ together with the NH to which it is bonded forms a morpholino group; and
  • R³ is H or NHR⁵
    • wherein R⁵ is
    • H, -quinolinyl or -isoquinolinyl,
    • —(CONH)_p phenyl wherein p is 0 or 1 and the phenyl is optionally substituted by one or more substituents independently selected from halogen, —C_1-6 alkyl, —C_1-6 haloalkyl, -morpholino, —SO₂NH₂, and methyl substituted benzothiazole.

The invention provides a method for preparing pinaverium bromide and an intermediate thereof, which relate to the pharmaceutical process. The cis isomer content of the intermediate 2-[2-(2-morpholino-2-ethoxy-ethyl)]-6, 6-dimethyl nopyl alkyl is high and the cis-isomer content of the obtained pinaverium bromide is more than or equal to 99 percent. The intermediate 2-[2-(2-morpholino-2-ethoxy-ethyl)]-6, 6-dimethyl nopyl alkyl is prepared by collecting cut fraction of 133-134 DEG C under 270 Pa.

The invention discloses a simple and efficient visual biosensing signal amplification method. The method comprises the following steps: binding a catalytic nano-material physically-embedded functionalized compound probe to the surface of a solid phase platform through a specific molecular recognition reaction by using a target analyte in a sample solution; changing the environmental factors of thesurface of the solid phase platform to release the catalytic nano-material from the functionalized compound probe into the solution; adding a weakly acidic 2-(N-morpholino)ethanesulfonic acid solution and a chloroauric acid solution to the obtained mixed solution to rapidly generate a red nano-gold solution, wherein the formation time of the red nano-gold solution is inversely proportional to theanalyte concentration; and performing signal reading by using a battery-powdered cheap and small timer to realize the low-cost portable quantitative detection of the analyte. The method has the advantages of simplicity in operation, low cost, no professional analytical instruments, and suitableness for onsite analysis and instant detection, and can be directly applied to fields of medical diagnosis, environmental monitoring, food safety and the like.