38 results about "Myonectin" patented technology

The invention relates to the field of biological medicine and cosmetics, in particular to laminin RL-20 and application of the laminin RL-20 in cosmetics.

The invention discloses recombinant laminin with anti-aging and repairing effects and a preparation method of the recombinant laminin, relates to the technical field of recombinant proteins, and aims to solve the technical problems that existing laminin is relatively low in yield and limited in application. According to the recombinant laminin with the anti-aging and repairing effects, the sequence of the recombinant laminin is shown as SEQ ID No.3, the total number is 363aa, and the predicted molecular weight is 40.4 kD. Compared with the prior art, the recombinant laminin is synthesized by embedding a laminin gene sequence into a pichia pastoris genome for heterologous expression. A cytological detection method for evaluating the efficacy of a skin care product is applied, and a research result shows that the recombinant laminin can remarkably promote cell adhesion and migration activity and can stimulate cells to generate collagen, so that the anti-aging and repairing functions of the recombinant laminin are shown.

The invention discloses a lung airway organoid culture system with a trachea branch morphological structure as well as application and a culture method of the lung airway organoid culture system, and relates to the technical field of lung airway organoid culture. The hydrogel culture system is prepared from I-type rat tail collagen, gelatin, hyaluronic acid, laminin, low-melting agarose, chondroitin sulfate, fibronectin, elastin, epidermal growth factors, fibronectin L and transglutaminase; the induction culture medium is prepared from a 1XB27 supplement, a fiber growth factor, glutamine, glutathione, A8301, SB202190, IBMX, 8-Br-cAMP, FGF7, FGF10, Shh and ascorbic acid. The culture system is clear in component, low in price and cost and simple and convenient in preparation method, more importantly, the lung airway organoid which is closer to the physiological state can be efficiently induced to be formed within a short time, and the generated organoid has a branch form similar to an in-vivo trachea and contains various types of functional airway epithelial cells at the same time; and a more preferable scheme is provided for constructing an airway organ with an airway branch form based on the adult lung stem cells.

Provided herein are antibodies or antigen-binding fragments thereof targeting Nectin-4. Also provided herein are the uses and methods of the antibodies or antigen-binding fragments thereof, or the antibody-drug conjugates in treating cancers.

The present disclosure provides conjugates of anti-Nectin-4 antibodies or antigen binding fragments thereof to a myeloid cell agonist, compositions comprising the conjugates, and methods of treating cancer with the conjugates. The present disclosure also provides for anti-Nectin-4 antibodies or antigen binding fragments thereof and method for using the antibodies or antigen binding fragments thereof in treating cancer.

A universal biological prosthesis of acellular dermal matrix (ADM) intended for use in body reconstruction processes and method of manufacture. A universal biological prosthesis of acellular dermal matrix (ADM) intended for use in body reconstruction processes, comprising: a first layer of ADM of biological origin arranged inferiorly to delimit a cavity with a lower edge; a filling material disposed in the cavity surrounded by the first layer of ADM consisting of a ground ADM having the consistency of a fibrous paste; and a second layer of ADM of biological origin arranged on the upper part covering the filling material and whose upper edge rests peripherally on the lower edge of the first layer of ADM, thus defining a rim;where the first layer of ADM and the second layer of ADM are joined by an eyebrow overlap along the upper edge of the first layer of ADM with the lower edge of the peripheral rim using a biocompatible adhesive, or by absorbable suture; where the first layer of ADM, the second layer of ADM and the ADM filling material are not immunogenic; and where the ADM comprises collagen fibers, fibronectin, elastin, laminin, glycosaminoglycans and hyaluronic acid. Method for its manufacture.

The invention relates to the technical field of artificial dermis, in particular to a preparation method and application of artificial dermis based on bionic acellular matrix. The artificial dermis comprises a bionic acellular matrix component A and an auxiliary material component B, wherein the component A comprises type I collagen, type VI collagen, elastin, fibronectin, laminin, mother cell protein and glucan; the component B comprises antibacterial peptide, antibiotics and a high-molecular polymer material; the mass ratio of the component A to the component B is (4-6): (1-4). The preparation method comprises the following steps: respectively preparing procedure mother liquor of the component A and the component B, preparing hydrogel with a double-layer gradient structure through step-by-step crosslinking, and then carrying out freeze drying and sterilization treatment. The artificial dermis disclosed by the invention has good biocompatibility and antibacterial property, can effectively promote wound healing, is suitable for repairing acute and chronic wounds of four limbs, faces and trunk parts, and is particularly suitable for repairing complex wounds with exposed bones, tendons, nerves and blood vessels.

The invention provides a serum-free medium for maintaining dryness of mesenchymal stem cells as well as a preparation method and application of the serum-free medium, and belongs to the technical field of biological medicines. The serum-free culture medium comprises a basic culture medium and an exogenous additive component, the basic culture medium is a DMEM / F12 culture medium, and the exogenous additive components comprise bFGF, EGF, PDGF-BB, recombinant human fibronectin, laminin, insulin, transferrin, sodium selenate, beta-mercaptoethanol and ascorbic acid. The serum-free culture medium provided by the invention is a special culture medium for the mesenchymal stem cells, which is completely free of animal-derived components, is suitable for large-scale amplification of clinical-grade cell treatment products, is relatively low in preparation cost and high in stem differentiation capability, and can remarkably improve the proliferation efficiency of the cultured mesenchymal stem cells.

Provided herein are peptides and conjugates, including radionuclide conjugates, useful in compositions and methods of treating, diagnosing, monitoring, and / or imaging a disease, disorder, or condition associated with expression of one or more targets, including Nectin-4.

The present application relates to the technical field of artificial dermis, and particularly relates to a preparation method and application of artificial dermis based on biomimetic acellular matrix. The artificial dermis comprises a biomimetic acellular matrix component A and an auxiliary component B, wherein the component A comprises type I collagen, type VI collagen, elastin, fibronectin, laminin, matricellular protein and dextran; the component B comprises antibacterial peptide, antibiotic and high molecular polymer material; the mass ratio of the component A to the component B is (4-6):(1-4). The preparation method comprises the steps of respectively preparing A and B component process mother liquor, step-by-step crosslinking to prepare a hydrogel with a double-layer gradient structure, and then freeze-drying and sterilization treatment. The artificial dermis has good biocompatibility and antibacterial performance, can effectively promote wound healing, and is suitable for repairing acute and chronic wounds of limbs, facial parts and trunk parts, and is particularly suitable for repairing complex wounds with exposed bones, tendons, nerves and blood vessels.

The application provides a multi-specific antibody monomer having a light chain (LC) and a heavy chain (HC), each having a N-terminal and a C-terminal, comprising, a first scFv domain having a binding affinity to a tumor-associated antigen (TAA) at the N-terminal of the HC (Position 1, or P1), a Fab domain having a binding affinity to CD3, and a second scFv domain having a binding affinity to PD-L1 at the C-terminal of the LC (Position 5, or P5) or the N-terminal of the LC (Position 6, or P6). In some embodiments, the TAA comprises EGFR, HER3, DLL3, HER2, GPC3, HLA-G, uPAR, Nectin4, Fra, Tissue Factor, B7-H4, VEGFR2, B7-H3, Muc16, CEACAM6, Claudin6, CLDN18.2, LGR5, GCC, CD20, CD19, Integrin β6, Muc1, CDH6, FAP, CAIX, Integrin β4, CDH17, Lewis B / Y, GPRC5D, CEACAM5, ROR1, or EGFR vIII.

The application discloses application of a separating enzyme inhibitor in preparation of an antitumor drug, and the separating enzyme inhibitor is toxoflavin and a derivative thereof. The compound can inhibit, at a molecular level, cleavage activity of an important proteinase, namely separating enzyme, on one subunit Scc1 of a mucin; at a cellular level, the toxoflavin derivative Walrycin B can inhibit proliferation of cancer cells such as human cervical cancer (HeLa), liver cancer (HepG2), breast cancer (MCF-7) and prostate cancer (PC3), and can arrest the cell cycle of the cancer cells at a G2 / M phase; and at an allogeneic transplantation tumor animal model, the Walrycin B can significantly inhibit growth of a mouse tumor.

The invention is directed to a composition of soluble placental ECM in storage solution and the methods of manufacture related thereto. The solubilized placental ECM solution is manufactured through a process of partitioning placental ECM from the placental tissue in a high acidic pH environment and performing an ultrafiltration step and diafiltration step through a tangential flow filtration system to increase the pH. The soluble placental ECM retains the native characteristics of placental ECM components and includes collagens (types I, III, V, VI, IX, and X), elastin, laminin, and fibronectin.

Disclosed herein are Nectin-4 antibodies. In some embodiments, the Nectin-4 antibodies can be used in treatments such as, for example, cancer treatments.

The invention provides a solution to deficiencies in innate immune cell targeting in solid tumors, including bladder cancer, through IL-18 or IL-15 Tetra protein targeted Killer Engagers (TetraKEs). These leverage CD16 and CD56 to enhance natural killer (NK) cell targeting of Nectin-4+ bladder tumor cells, offering a novel immunotherapeutic approach.

The invention relates to the technical field of biomedical materials, and discloses a freeze-dried matrigel and a preparation method and application thereof, and the freeze-dried matrigel is prepared by dissolving a natural basement membrane extract in a high-glucose DMEM (Dulbecco Modified Eagle Medium); the natural basement membrane extract is derived from EHS mouse tumor tissue rich in extracellular matrix proteins. The freeze-dried matrigel provided by the invention is prepared by taking a natural basement membrane extract as a core active component through an optimized freeze-drying process. After redissolution, a three-dimensional fiber network structure can be quickly recovered, the biological functions of active ingredients such as collagen IV, laminin and nest protein in the basement membrane are reserved, and the storage stability is high; meanwhile, the matrix gel has the advantages of good redissolution effect and good cell compatibility, can be widely applied to the fields of cell culture and in-vitro disease model construction, and solves the technical problems that the traditional liquid matrix gel needs to be stored at low temperature, is high in transportation cost and is easy to degrade and inactivate.

The invention relates to a material containing an acellular matrix and a preparation process thereof, and belongs to the technical field of biological materials. The decellularized matrix adopts ammonium hydroxide to crack lipid, Triton X-100 membrane removal protein and nuclease to degrade nucleic acid to thoroughly eliminate immunogenicity and synchronously reserve bioactive molecules such as type I collagen, growth factors and the like, a porous structure is constructed by combining supercritical CO2 degreasing and freeze-drying technologies, and the porous structure can be used for preparing the biomedical material. And the degradation rate is regulated and controlled by matching the natural restriction enzyme cutting sites with the hydrolysis characteristics of the silk fibroin. The hydrogel system takes an oxidized hyaluronic acid-thiolated chitosan dynamic covalent bond network as a core, the tensile strength is enhanced after silk fibroin beta-folding reinforcement, the hydrogel system has the lubricity of hyaluronic acid and the broad-spectrum antibacterial property of chitosan / N-sodium lauroyl sarcosinate, and amino-RGD-laminin triple cell adhesion sites promote ECM deposition; furthermore, nano calcium phosphate particles are compounded to form a nano reinforced interface, so that the mechanical property and the antibacterial property of the material are remarkably improved.

To provide a novel composition for application on the skin of human or the like that prevents the deterioration of skin function.SOLUTION: The present invention provides a composition that comprises tranexamic acid for inhibiting the degradation of laminin 511 in the skin.SELECTED DRAWING: None

The present invention provides antigen-binding proteins capable of binding to Nectin-4 polypeptides conjugated to chemotherapeutic agents, for use in increasing sensitivity of tumors to the chemotherapeutic agents, for use in the treatment of cancers characterized by Nectin expressing tumor cells. In one embodiment, the present invention provides conjugates of an anti-Nectin-4 antibody to a camptothecin analogue, such as exatecan or SN-38, through a cleavable linker.

The present application relates to a kind of laminin or its specific fragment polypeptide, the complex formed by non-covalent adsorption immunological factor with it and its application in biomaterials.38 polypeptides screened by the present application. One or more of the complete laminin containing the polypeptide screened by the present application, or complete fibronectin is used as carrier protein, and is mixed with biomaterial base, immunological factor etc. to make complex, because there is non-covalent adsorption between carrier protein and immunological factor, immunological factor can be biomimetic adsorption carried on the material combined with carrier protein. The product containing the complex of the present application can automatically dissociate and release immunological factor in neutral environment and maintain the slow release for several days.Moreover, the product made of the complex of the present application can avoid activating the immune response of the implant, solve the problem that transgenic expression vector enters the patient's body and easily induces intense immune response, difficult to realize secondary administration etc.

The invention discloses a specific skeletal muscle cell targeting liposome and a preparation method thereof, and belongs to the technical field of skeletal muscle tissue targeting. The lipidosome specifically targeting the skeletal muscle cells comprises a lipidosome body, and the surface of the lipidosome body is modified with a peptide fragment specifically bound by polyethylene glycol-amino acid containing an acidic side chain and biotin-streptavidin-laminin. The lipidosome for specifically targeting the skeletal muscle cells can be enriched at skeletal muscle tissues, and the coated recombinant adeno-associated virus can be efficiently released around the skeletal muscle cells.

The invention relates to the field of biological medicine and cosmetics, in particular to laminin polypeptide GF-13 and application thereof.

Provided are an anti-Nectin-4 antibody and the use thereof. The anti-Nectin-4 antibody or antigen-binding fragment can specifically bind to Nectin-4 and has high affinity and strong endocytosis capacity, and therefore can be used for diagnosis, prevention, and / or treatment of tumors (comprising cancers and benign tumors).

To find out a new function of Myonectin and to provide a suitable use thereof.SOLUTION: The myonectin production promoter is contained in a composition for inhibiting IL-1 α expression. The composition is preferably in the form of an external preparation for skin or a food or drink.SELECTED DRAWING: Figure 1

Disclosed herein are an anti-Nectin-4 antibody, an antibody-drug conjugate thereof, and use thereof. The anti-Nectin-4 antibody and the antibody-drug conjugate thereof can be used for treating a disease.

A tricyclic polypeptide conjugated drug and the use thereof are provided. Specifically provided are a compound as represented by formula (III), and a pharmaceutically acceptable salt thereof. A pharmaceutical composition comprising a therapeutically or prophylactically effective amount of the compound or the pharmaceutically acceptable salt thereof is further provided. The compound or the pharmaceutically acceptable salt thereof and the pharmaceutical composition can be used in the preparation of a drug for treating Nectin-4 overexpressed solid tumors.

The present invention relates to a serum-free medium for adherent cell culture, comprising: a serum substitute comprising amino acids, lipids, vitamins and hormone proteins; the lipid comprises at least one of palmitic acid, arachidonic acid, cholesterol, vitamin E acetate, linoleic acid, linolenic acid, oleic acid, stearic acid, palmitoleic acid and octadecanoic acid; the vitamins comprise at least one of folic acid, vitamin C, vitamin E, vitamin B1, nicotinamide, inositol and calcium pantothenate; the hormone proteins comprise at least one of recombinant insulin, recombinant transferrin, recombinant albumin, recombinant epidermal growth factors and recombinant basic fibroblast growth factors, and at least one of recombinant laminin, recombinant IV type collagen and recombinant heparan sulfate proteoglycan. The serum-free culture medium can promote proliferation and differentiation of adherent cells, and the repeatability and accuracy of experiments are improved.

The present invention provides methods for identifying or selecting cancer patients for treatment with a Nectin-4 targeted therapeutic on the basis of the patients having a Nectin-4 copy number of 4 or more.

The objective is to provide therapeutic drugs that promote wound healing. [Solution] Analysis of Dectin-1 gene-deficient mice revealed that inhibiting Dectin-1 promotes wound healing caused by burns. When laminarin, a Dectin-1 antagonist, or barley β-glucan 30sp was administered to the wound, a significant wound healing promoting effect was observed. Since low molecular weight β-glucan, a Dectin-1 antagonist, has the effect of promoting wound healing, a wound healing promoter containing it as an active ingredient can be provided.

The present invention relates to a camelina cake extract and a composition comprising said extract, for a cosmetic treatment. The extract according to the invention has anti-aging, propigmenting and moisturising effects, by acting on the production of various molecules such as hyaluronic acid, collagen, laminins and melanin, and on the inhibition of oxidation, radicals and glycation.