This useful solution refers to a process for fabricating multilayer
polymer nanofibers (4
layers) CPPCBR from
chitosan (CS),
polyethylene glycol (PEG), and
polylactic acid (PLA) polymers, imbued with a mixture of three active ingredients:
curcumin (Cur) extracted from turmeric (
Curcuma longa),
berberine (Ber) extracted from Coscinium fenestratum, and rotundin (Rot) extracted from
Stephania sp., using
electrospinning combined with
gelatin as a binder, performed on a CHITEC 3D printer. CPPCBR multilayer
polymer nanofibers are composed of non-toxic, biodegradable, and highly
biocompatible polymers:
chitosan,
polyethylene glycol, and
polylactic acid, bonded with a mixture of three active ingredients Cur / Ber / Rot (weight ratio 50:10:40), with a total content of 9% of the three active ingredients in the
fiber. CPPCBR multilayer
polymer nanofibers (4
layers) can carry four times more active ingredients and provide better release of active ingredients compared to single-layer nanofibers of the same composition. Furthermore, the multilayer structure of CPPCBR multilayer polymer nanofibers obtained from the utility solution process allows for slower and more stable release of the
active ingredient compared to single-layer
nanofiber structures.