The invention relates to the technical field of nuclear power station heat insulation systems, in particular to a sampling method for quality inspection of a primary side end sealing partition plate of a nuclear power steam generator. According to the sampling method, various production factors and application conditions in the sampling process can be sufficiently considered, the overall quality of the primary side end sealing partition plate of the steam generator can be comprehensively inspected by virtue of hardness uniformity test, chemical component uniformity test, normal-temperature tensile test, high-temperature tensile test, room-temperature impact test, hardness test, grain size detection, distribution of carbides, nonmetal impurity analysis, and chemical analysis of finished products of the primary side end sealing partition plate of the steam generator, the production capability and management capability of a manufacturer can be verified, whether the quality of the primaryside end sealing partition plate of the steam generator meets the design and nuclear security requirement or not can be judged, the repeatability of the production quality can be ensured, the inspection items are effectively reduced, and the inspection cost is maximally decreased.