The utility model relates to the technical field of display screen detection, and provides a display screen load weighing detection device which comprises a base, sliding grooves are formed in the two sides of the top end face of the base, a connecting groove is formed in the center of the base, and the two sides of the connecting groove are communicated with the interiors of the two sliding grooves respectively. Sliding blocks are slidably mounted at the two ends of the sliding groove correspondingly, a connecting block is welded to the center of the connecting groove, and screw rods are arranged on the two sides of the connecting block correspondingly. According to the display screen load weighing detection device, a display screen can be supported and fixed through clamping plates, a motor drives a screw rod to rotate, the distance between the two clamping plates can be adjusted under the action of a movable block, a sliding block and a sliding groove, then display screens of different sizes can be supported, a clamping plate can be pushed through a telescopic rod to clamp the display screen, and the display screen load weighing detection device is convenient to use. The fixing effect can be achieved, load weighing detection can be carried out on display screens of different sizes, and it is avoided that the display screens deviate, and the accuracy of load weighing detection is affected.