The invention provides nutritious beef jerky and a preparation method thereof and relates to the technical field of food processing. The nutritious beef jerky is mainly prepared from, by weight, 150-200 parts of beef, 40-60 parts of curing materials, 20-30 parts of passion fruit, 50-80 parts of Fructus Crataegi, 10-15 parts of Flos Jasmini Sambac, 3-9 parts of Fructus Forsythiae, 3-9 parts of Herba Menthae, 5-10 parts of Herba Rabdosiae Serrae, 1-3 parts of dry red pepper, 2-5 parts of garlic, 2-7 parts of ginger, 5-10 parts of olive oil and an appropriate amount of water. The nutritious beef jerky and the preparation method thereof have the advantages that a specific formula is combined with the special preparation method to make the food materials give maximal play to efficacy, and accordingly the prepared beef jerky exudes a mouthwatering aroma, is fluffy with elasticity, smooth, tender with a crispy crust and delicious, cannot make people get inflamed after being eaten, and is free of harmful substances such as pigments and preservatives, thereby being safe and healthy and worthy of popularization and production.