The invention discloses a method for extracting enteromorpha
polysaccharide by using an ultrasonic-assisted deep-eutectic
solvent, which comprises the following steps: rinsing
silt on the surface of fresh enteromorpha with clear water,
drying, freeze-
drying, crushing, filling enteromorpha
powder into a sealed bag, and refrigerating in a refrigerator for later use; the preparation method comprises the following steps: by taking 1, 2-
propylene glycol as a
hydrogen bond donor and
choline chloride as a
hydrogen bond
receptor, adding deionized water to prepare a deep-eutectic
solvent; the method comprises the following steps: weighing enteromorpha
powder, mixing with a
deep eutectic solvent, carrying out water bath and ultrasonic-assisted extraction, and centrifuging to obtain an enteromorpha
polysaccharide leaching solution; and adding absolute ethyl
alcohol into the enteromorpha
polysaccharide extract, uniformly mixing, standing at 4 DEG C for 12 hours, centrifuging, taking precipitate, redissolving, carrying out rotary
evaporation on the precipitate, deproteinizing by adopting a Sevage method, dialyzing, decoloring, taking supernate, and freeze-
drying, so as to obtain the enteromorpha polysaccharide. The method disclosed by the invention has the advantages of high efficiency, short time consumption, high yield, simplicity in operation and the like, is mild in extraction condition, safe, green and environment-friendly, and lays a foundation for extraction of enteromorpha polysaccharide in
food industry.