The invention provides filament yarn bonding type honeycomb structure high-elastic antibiosis non-woven cloth. The filament yarn bonding type honeycomb structure high-elastic antibiosis non-woven cloth comprises a plurality of synthetic fiber nets sequentially stacked in a reciprocating Z shape, an antibacterial agent is sprayed inside the synthetic fiber nets, the synthetic fiber nets are sequentially connected in an end-to-end mode, the fiber net included angle between the adjacent synthetic fiber nets is an acute angle smaller than 2 degrees, hot melting filament yarns are arranged on the front surfaces and the back surfaces of the synthetic fiber nets in the width directions of the synthetic fiber nets at equal intervals in a side by side mode, and the hot melting filament yarns are folded in the reciprocating Z shape with the synthetic fiber nets. Each synthetic fiber net is a layer-shaped fiber net and formed by overlapping an upper layer fiber net, an elastic fiber net and a lower layer fiber net. The preparation method comprises the following steps that 1, preparation before net forming is conducted; 2, machinery carding for net forming is conducted; 3, fiber net synthetization is conducted; 4, the antibacterial agent is sprayed, 5, the nets are vertically laid; 6, drying is conducted; 7, edge cutting is conducted; 8, winding is conducted. The filament yarn bonding type honeycomb structure high-elastic antibiosis non-woven cloth has the advantages that not only is the high elasticity achieved, the antibacterial performance good, the structure simple, but also the cost is low, and the environment protection is achieved.