The invention relates to the technical field of fabrics, in particular to an antibacterial comfortable fabric and a preparation process thereof. The antibacterial comfortable fabric is formed by weaving composite yarns, wherein the composite yarns are formed by stacking surface yarns, elastic fiber yarns and bottom yarns; after stacking forming, the surface yarns form a surface layer, the elasticfiber yarns form an elastic middle layer, and the bottom yarns form a bottom layer; the elastic fiber yarns are low-temperature spandex with a fineness of 30 to 60 D; and the bottom yarns are corn fibers with a fineness of 45-100D / 45-75F. The antibacterial comfortable fabric disclosed by the invention is formed by compositely weaving three kinds of yarn fibers, three yarn layers are sequentially formed on the prepared fabric, and the middle layer is elastic, so the fabric is improved in wearing comfort and skin friendliness, has environmental protection, warm keeping, machine washing, non-ironing functions and the like, and is good in air permeability, high in moisture volatilization rate, good in quick-drying property, comfortable to wear and free of stuffiness and skin sticking feeling;and meanwhile, the corn fibers are adopted as the bottom layer, so the contact comfort of the fabric and the skin is improved.