The invention relates to the technical field of steel structures, in particular to an adjustable building stand column connecting mechanism and a using method. The adjustable building stand column connecting mechanism comprises a first column body and a second column body, and further comprises a controller, a first adjusting device, a second adjusting device and a top connecting device; and the first adjusting device is arranged at the bottom of the first column body and used for accurately adjusting the installation position of the first column body, the first adjusting device comprises a bottom shell, the second adjusting device comprises a top cover, a bottom cover, four flexible connecting assemblies and four rigid connecting assemblies, the top connecting device comprises a lining and a connecting assembly, the lining is arranged on the outer wall of the top of the second column body, the connecting assemblies are arranged on the top of the lining, and the rigid connecting assemblies are electrically connected with the controller. According to the adjustable building stand column connecting mechanism and the using method, the mounting position of stand columns can be accurately adjusted, and time and labor are relatively saved during adjustment; and meanwhile, according to the stress condition of the stand columns, an internal connecting mechanism can be automatically adjusted to prevent collapse.