The invention relates to a preparation method of a three-yellow chicken feedstuff. The feedstuff is composed of momordin powder, hyacinth bean protein powder, black soybean protein powder, animal protein powder, bloodworm powder, wax gourd powder, plant powder, corn flour, fish meal, pumpkin powder, indigestion tablets, white fish meal, common salt, dandelion powder, Asiatic plantain seed powder, salvia miltiorrhiza powder, chicken gizzard membrane powder, and cyperi rhizome powder. The components are blended according to weight percentages. The three-yellow chicken feedstuff has advantages of low cost, available materials, simple formula, easy preparation, and good breeding effect. The bred three-yellow chickens have advantages of fast growth, robust and beautiful build, bright feather color, big size, and low disease incidence. With traditional individual breeding conditions and large-scale breeding conditions, due to nutritional depletion, three-yellow chickens have defects of featherless, feather pecking, dim feather color, low growth, small size, light weight, and loose and messy feather. The chickens have less activity amount, and are easy to take a disease and to die. With the feedstuff provided by the invention, the problems can be effectively solved.